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<p>Cucumber mosaic virus (CMV) is a major plant pathogen with a wide host range and global distribution causing significant impact in many horticultural crops. During a diagnostic survey, <i>Adenium obesum</i> plants were found with mosaic, puckering and flower malformation symptoms. Serological assay (TAS-ELISA) and RT-PCR confirmed CMV infection. The coat protein (CP), movement protein (MP), and 2b genes of the virus isolate were cloned and sequenced. Sequence analysis revealed high similarity with subgroup IB isolates, showing 98–99% identity with Taiwanese and Brazilian isolates. Phylogenetic analyses further confirmed the placement of the Adenium isolate within subgroup IB, distinct from subgroups IA and II. Host range studies demonstrated systemic infection in <i>Nicotiana glutinosa</i>, <i>Vigna unguiculata</i> and <i>Phaseolus vulgaris,</i> producing characteristic mosaic and necrotic symptoms. This study provides the first molecular evidence of CMV infecting <i>A. obesum</i> in India and highlights its potential as a reservoir host.</p>

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Identification and partial characterization of cucumber mosaic virus in Adenium obesum: a new reservoir host in India

  • 期刊论文

  • 发表日期：

    2026年3月17日

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  • Y. Durga,
  • J. Vinodhini,
  • Ruth Ch,
  • K. Gopal,
  • C. Anuradha,
  • Marimuthu Somasundaram Saraswathi,
  • Nagendran Krishnan,
  • R. Selvarajan

摘要

Cucumber mosaic virus (CMV) is a major plant pathogen with a wide host range and global distribution causing significant impact in many horticultural crops. During a diagnostic survey, Adenium obesum plants were found with mosaic, puckering and flower malformation symptoms. Serological assay (TAS-ELISA) and RT-PCR confirmed CMV infection. The coat protein (CP), movement protein (MP), and 2b genes of the virus isolate were cloned and sequenced. Sequence analysis revealed high similarity with subgroup IB isolates, showing 98–99% identity with Taiwanese and Brazilian isolates. Phylogenetic analyses further confirmed the placement of the Adenium isolate within subgroup IB, distinct from subgroups IA and II. Host range studies demonstrated systemic infection in Nicotiana glutinosa, Vigna unguiculata and Phaseolus vulgaris, producing characteristic mosaic and necrotic symptoms. This study provides the first molecular evidence of CMV infecting A. obesum in India and highlights its potential as a reservoir host.