<p>Methyl bromide (MB) is being phased out due to its impact on the ozone layer and fumigant alternatives are needed for treatment of oak (<i>Quercus</i> spp.) logs infested with the oak wilt fungus, <i>Bretziella fagacearum</i>. Exportation of oak logs from the United States often requires phytosanitary measures to prevent the spread of <i>B. fagacearum</i>, which currently exists only in North America. Development of phytosanitary treatments is challenging in part due to obtaining and treating infested material that is representative of oak logs. A laboratory method to infest and treat oak wood would reduce the time and expense required to artificially infest or monitor progression of trees naturally infested with oak wilt to obtain material for large-scale treatments. We developed a method to infest red oak (<i>Quercus rubra</i>) blocks with <i>B. fagacearum</i> to test treatment efficacy of MB and ethanedinitrile (EDN). Oak blocks with intact bark were infested by applying a <i>B. fagacearum</i> spore suspension to exposed wood surfaces. Infested blocks were fumigated for 72&#xa0;h with 240&#xa0;g/m³ MB (<i>n</i> = 16), the current treatment for export oak logs, or 120&#xa0;g/m³ EDN (<i>n</i> = 24). <i>Bretziella fagacearum</i> was successfully recovered from all unfumigated control blocks (<i>n</i> = 16), allowing for comparison of treatment efficacy. Methyl bromide treatments were completely successful in eliminating <i>B. fagacearum</i> from treated blocks. Ethanedinitrile treatments greatly reduced recovery of <i>B. fagacearum</i>, but did not eradicate the pathogen completely. Development of this method will aid future research to develop effective treatments against the oak wilt pathogen.</p>