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<p>‘<i>Candidatus</i> Liberibacter asiaticus’ (<i>C</i>Las), the prevalent agent associated with Huanglongbing (HLB), poses a severe threat to global citrus production. This study aimed to investigate the occurrence of psyllid species (other than <i>Diaphorina citri</i>) landing on citrus trees, evaluate their survival on citrus over optimal acquisition access periods (AAP), assess their ability to acquire <i>C</i>Las, and infer their potential role in pathogen transmission. Field surveys were conducted in <i>Citrus sinensis</i> plants located in backyards and poorly managed groves in two locations of São Paulo State, Brazil. Psyllids from fifteen genera were identified, with <i>Isogonoceraia divergipennis</i> and <i>Heteropsylla cubana</i> being among the most constant or frequent species, showing greater abundance particularly in late winter and early spring. Controlled acquisition assays demonstrated that <i>I. divergipennis</i>, <i>H. cubana</i>, and <i>Triozoida limbata</i> could acquire <i>C</i>Las following a 72-h AAP, post-acquisition latency of 14 days and a subsequent 72-h post-latency period, with acquisition rates of 8.1%, 3.2%, and 2.4%, respectively, compared to 85.7% in <i>D. citri.</i> Notably, <i>C</i>Las was detected in the ‘head-mesothorax’ region only in <i>T. limbata</i> and <i>D. citri</i>, suggesting potential bacterial presence in the salivary glands and possible transmission competence. <i>C</i>Las titers were consistently higher in the ‘metathorax-abdomen’ than in the ‘head-mesothorax’. While the data indicate occasional <i>C</i>Las acquisition by psyllids other than <i>D. citri</i>, there is currently no evidence supporting their role as natural vectors. Nonetheless, these species may serve as valuable models for elucidating transmission barriers in non-competent psyllids and highlight the need for further investigation into their epidemiological relevance.</p>

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Acquisition of Candidatus Liberibacter asiaticus by psyllid species transiently landing and feeding on Citrus sinensis

  • 期刊论文

  • 发表日期：

    2025年11月11日

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  • Mayerli Tatiana Borbón-Cortés,
  • João Roberto Spotti Lopes

摘要

‘Candidatus Liberibacter asiaticus’ (CLas), the prevalent agent associated with Huanglongbing (HLB), poses a severe threat to global citrus production. This study aimed to investigate the occurrence of psyllid species (other than Diaphorina citri) landing on citrus trees, evaluate their survival on citrus over optimal acquisition access periods (AAP), assess their ability to acquire CLas, and infer their potential role in pathogen transmission. Field surveys were conducted in Citrus sinensis plants located in backyards and poorly managed groves in two locations of São Paulo State, Brazil. Psyllids from fifteen genera were identified, with Isogonoceraia divergipennis and Heteropsylla cubana being among the most constant or frequent species, showing greater abundance particularly in late winter and early spring. Controlled acquisition assays demonstrated that I. divergipennis, H. cubana, and Triozoida limbata could acquire CLas following a 72-h AAP, post-acquisition latency of 14 days and a subsequent 72-h post-latency period, with acquisition rates of 8.1%, 3.2%, and 2.4%, respectively, compared to 85.7% in D. citri. Notably, CLas was detected in the ‘head-mesothorax’ region only in T. limbata and D. citri, suggesting potential bacterial presence in the salivary glands and possible transmission competence. CLas titers were consistently higher in the ‘metathorax-abdomen’ than in the ‘head-mesothorax’. While the data indicate occasional CLas acquisition by psyllids other than D. citri, there is currently no evidence supporting their role as natural vectors. Nonetheless, these species may serve as valuable models for elucidating transmission barriers in non-competent psyllids and highlight the need for further investigation into their epidemiological relevance.