Scholarlink

<p><i>Plutella xylostella</i> (Linnaeus, 1758) (Lepidoptera: Plutellidae) is a phytosanitary problem for Brassicaceae culture in Brazil and other countries. Several studies reported the use of plants with insecticide action to reduce the pest dissemination and avoid the use of pesticides in crops. Annonaceae is a group of plants with insecticide potential mediated by compounds such as acetogenins that may corroborate the practices of pest integrated management. The aim of this work was to study the insecticidal and phytochemical potential of <i>Annona mucosa</i> (Annonaceae) extract in the control of <i>P. xylostella</i>. The crude extract of <i>A. mucosa</i> seeds was obtained by ethanolic maceration followed by rotoevaporation, and subsequently fractionated through liquid–liquid extraction, silica column chromatography, and medium-pressure liquid chromatography (MPLC). The insecticide bioassay applied on neonate larvae of <i>P. xyslostella</i> conducted in the laboratory under conditions of 25 ± 2&#xa0;°C of temperature, relative humidity of 67 ± 2% and photoperiod (12L:12D), with the crude extract and the fractions from the liquid–liquid extraction, silica filtration and MPLC. The identification and isolation of acetogenin in the purified fractions were conducted by thin layer chromatography (TLC), high performance liquid chromatography (HPLC) and NMR 1H and 13C. The results of the insecticide action of the crude extract allowed estimating the lethal concentrations LC<sub>50</sub> and LC<sub>99</sub>, being respectively 0.004 and 0.06%. All fractions of liquid–liquid extraction caused mortality of larvae (hexane 81.0%, acetate 83.0% and chloroform 89.0%), as well as hexane: chloroform fractions (1:1) and acetate from silica filtration, with 74.0 and 65.0%, respectively. All fractions from MPLC HEX:CHCl<sub>3</sub> (1:1) also caused larval mortality (LC1 = 62.0%; LC12 = 60.0%; 7–9 = 64.0%; 14 = 55.0%; 15–20 = 50.0%; 22–27 = 59.0%; 28–38 = 52.0% and 39–45 = 72.0%). In hexane: chloroform fractions (1:1) 1–32 acetogenin squamocin was identified and isolated in <i>A. mucosa</i>. Therefore, <i>A. mucosa</i> has insecticide action against neonatal larvae of <i>P. xylostella</i>.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Toxicity and bioassay-guided phytochemical study of Annona mucosa Jacq. (Annonaceae) on Plutella xylostella (Linnaeus, 1758) (Lepidoptera: Plutellidae)

  • 期刊论文

  • 发表日期：

    2025年11月24日

查看全文
  • Emerson dos Santos Ferreira,
  • Antônio Euzébio Goulart Sant’Ana,
  • Aldenir Feitosa dos Santos,
  • Eurico Eduardo Pinto de Lemos,
  • Roseane Cristina Predes Trindade

摘要

Plutella xylostella (Linnaeus, 1758) (Lepidoptera: Plutellidae) is a phytosanitary problem for Brassicaceae culture in Brazil and other countries. Several studies reported the use of plants with insecticide action to reduce the pest dissemination and avoid the use of pesticides in crops. Annonaceae is a group of plants with insecticide potential mediated by compounds such as acetogenins that may corroborate the practices of pest integrated management. The aim of this work was to study the insecticidal and phytochemical potential of Annona mucosa (Annonaceae) extract in the control of P. xylostella. The crude extract of A. mucosa seeds was obtained by ethanolic maceration followed by rotoevaporation, and subsequently fractionated through liquid–liquid extraction, silica column chromatography, and medium-pressure liquid chromatography (MPLC). The insecticide bioassay applied on neonate larvae of P. xyslostella conducted in the laboratory under conditions of 25 ± 2 °C of temperature, relative humidity of 67 ± 2% and photoperiod (12L:12D), with the crude extract and the fractions from the liquid–liquid extraction, silica filtration and MPLC. The identification and isolation of acetogenin in the purified fractions were conducted by thin layer chromatography (TLC), high performance liquid chromatography (HPLC) and NMR 1H and 13C. The results of the insecticide action of the crude extract allowed estimating the lethal concentrations LC50 and LC99, being respectively 0.004 and 0.06%. All fractions of liquid–liquid extraction caused mortality of larvae (hexane 81.0%, acetate 83.0% and chloroform 89.0%), as well as hexane: chloroform fractions (1:1) and acetate from silica filtration, with 74.0 and 65.0%, respectively. All fractions from MPLC HEX:CHCl3 (1:1) also caused larval mortality (LC1 = 62.0%; LC12 = 60.0%; 7–9 = 64.0%; 14 = 55.0%; 15–20 = 50.0%; 22–27 = 59.0%; 28–38 = 52.0% and 39–45 = 72.0%). In hexane: chloroform fractions (1:1) 1–32 acetogenin squamocin was identified and isolated in A. mucosa. Therefore, A. mucosa has insecticide action against neonatal larvae of P. xylostella.