<p>Tobacco (<i>Nicotiana tabacum</i>) is cultivated in over 120 countries, with an estimated global production of approximately 6.2 million tons annually. The Virginia variety, due to its high nicotine content, plays a central role in both international markets and domestic industrial processing. Among the pathogens affecting this crop, plant-parasitic nematodes are a significant threat to yield and quality. The false root-knot nematode (<i>Nacobbus</i> spp.) has been reported in association with tobacco; however, its impact on yield losses remains undocumented, and its interaction with different varieties is still poorly understood. This study presents a detailed histopathological analysis of Virginia tobacco (cv. K394) roots naturally infected with <i>N. celatus,</i> collected from northwestern Argentina. Healthy and galled roots were fixed, processed and examined using conventional light microscopy techniques. Syncytia, induced by the female nematodes within the central cylinder, were composed of hypertrophic cells with dense cytoplasm. Nematode colonization resulted in extensive disruption of vascular tissues, cambium, and cortical parenchyma. Additionally, non-functional syncytia associated with fungal hyphae and filiform nematodes were observed. These findings provide the first anatomical characterization of the <i>N. celatus</i>-tobacco interaction and highlight key aspects of the nematode’s life cycle, including potential synergistic interactions with soilborne fungi that may intensify host tissue damage. </p>