Evaluating of nano-silver and lavender (Lavandula angustifolia Mill) extract efficacy against Tetranychus urticae Koch (Acari: Tetranychidae)
摘要
The use of nanoparticles from plants associated with biopesticides is gaining an increasingly important in agriculture. Tetranychus urticae Koch (Acari: Tetranychidae) is an aggressive, invasive species that is harmful to park, forest, ornamental and many cultivated plants. In this study, silver nanoparticles (AgNPs) were synthesized using Lavandula angustifolia Mill water extract. The synthesized AgNPs was identified by Ultraviolet–Visible (UV–Vis) spectrophotometer, Fouirer Transform-Infrared Spectroscopy (FTIR), Scanning Electron Microscope, X-Ray Diffraction techniques. AgNPs exhibited a maximum UV–vis absorbance at 480 nm. Hydroxyl group was observed at 3262.97 cm−1in FTIR spectrum. AgNPs were found to be spherical and less than 50 nm in diameter.The acaricidal effect L. angustifolia of ethanol extract and AgNPs was investigated using leaf dipping method. Experiments were carried out using (3 cm diameter) leaf disks of Phaseolus vulgaris L.angustifolia ethanol extract and silver nanoparticles (AgNP) were compared in 4 different concentrations. The acaricidal effect of ethanol extract was found as 95.46% and 62.24% at the concentration of 12% and 6% for 5 days respectively. AgNPs effect was observed as 98.82% at 336 ppm at the same period. All the extracts exhibited significant mite mortality as compared to control also, AgNPs were the most toxic, based on the LC50 and LC90 values, against the T. urticae compared to the other extracts tested. As a result, AgNPs used in this study are thought to be used as an alternative method to pesticides for control of T. urticae. However, the impact of these AgNPs effect on T. urticae should also be needed further studies.