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<p>Advances in chemical synthesis have significantly reduced the cost of producing synthetic bioactive peptides, paving the way for new antimicrobial agents that protect human health, agriculture and livestock from the growing threat of antimicrobial resistance. This study investigated the in vivo and in vitro inhibitory activity of the synthetic peptide <i>Ca</i>Def2.1<sub>G27-K44,</sub> bioinspired by a region of a natural defensin called <i>Ca</i>Def2.1, over <i>Xanthomonas euvesicatoria</i>, an important pathogen of pepper crops. The <i>Ca</i>Def2.1<sub>G27-K44</sub> peptide showed a minimum bactericidal concentration (MBC) of 5&#xa0;µM against <i>X. euvesicatoria,</i> with a minimum exposure time of 3&#xa0;h for inoculum viability loss, and caused permeabilization of the cytoplasmic membrane. Growth control assays of <i>X. euvesicatoria</i> by <i>Ca</i>Def2.1<sub>G27–K44</sub> were conducted under greenhouse conditions, and the method of bacterial infiltration inoculation on leaves was employed. The concentrations used were MBC and 2 × MBC. The results revealed a visible reduction in symptoms in leaves treated with <i>Ca</i>Def2.1<sub>G27-K44</sub> and that the peptide did not present toxicity to the plant. These findings suggest potential applications of <i>Ca</i>Def2.1<sub>G27–K44</sub> in phytopathogen control in agriculture.</p>

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The potential of the CaDef2.1G27-K44 bioinspired peptide to control bacterial disease in Capsicum with a focus on Xanthomonas euvesicatoria

  • 期刊论文

  • 发表日期：

    2025年7月17日

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  • Larissa Maximiano Resende,
  • Érica de Oliveira Mello,
  • Gabriel Bonan Taveira,
  • Anderson Yusei Suzuki Fukuji,
  • Antônio André da Silva Alencar,
  • Claudia Pombo Sudré,
  • Rosana Rodrigues,
  • Andre de Oliveira Carvalho,
  • Katia Valevski Sales Fernandes,
  • Valdirene Moreira Gomes

摘要

Advances in chemical synthesis have significantly reduced the cost of producing synthetic bioactive peptides, paving the way for new antimicrobial agents that protect human health, agriculture and livestock from the growing threat of antimicrobial resistance. This study investigated the in vivo and in vitro inhibitory activity of the synthetic peptide CaDef2.1G27-K44, bioinspired by a region of a natural defensin called CaDef2.1, over Xanthomonas euvesicatoria, an important pathogen of pepper crops. The CaDef2.1G27-K44 peptide showed a minimum bactericidal concentration (MBC) of 5 µM against X. euvesicatoria, with a minimum exposure time of 3 h for inoculum viability loss, and caused permeabilization of the cytoplasmic membrane. Growth control assays of X. euvesicatoria by CaDef2.1G27–K44 were conducted under greenhouse conditions, and the method of bacterial infiltration inoculation on leaves was employed. The concentrations used were MBC and 2 × MBC. The results revealed a visible reduction in symptoms in leaves treated with CaDef2.1G27-K44 and that the peptide did not present toxicity to the plant. These findings suggest potential applications of CaDef2.1G27–K44 in phytopathogen control in agriculture.