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<p>Sunflowers are among the world’s most widely known oilseed and ornamental crops which can be infected by numerous viruses, leading to yield losses. This study surveyed and collected sunflower leaves showing virus-like symptoms such as mosaic, mottle, and chlorotic spots from different regions of Thailand during 2023–2024 to detect causative viruses. Three target viruses were investigated consisting of sunflower mosaic virus (SuMV) (proposed name <i>Potyvirus helitessellati</i>), sunflower mild mosaic virus (SuMMoV) (proposed name <i>Potyvirus helitenuitessellati</i>), and sunflower chlorotic mottle virus (SuCMoV) (proposed name <i>Potyvirus helichloromaculae</i>). A multiplex reverse transcription polymerase chain reaction (RT-PCR) assay was developed for simultaneous detection of mixed virus infections. The annealing temperature was optimized for multiplex RT-PCR amplification, along with sensitivity and specificity assays. A total of 190 sunflower samples from 22 growing areas in 12 provinces across 4 regions of Thailand were subjected to multiplex RT-PCR detection. Single infection of SuCMoV was most frequently detected, followed by SuMV and SuMMoV. Among mixed infections, SuMV and SuCMoV co-infection was the most common, and infections with all three viruses were also detected. Individual viruses were isolated and mechanically inoculated onto sunflowers and other plants to fulfill Koch’s postulates, revealing that all viruses caused distinct symptoms in sunflowers similar to field observations. Phylogenetic and sequence analysis revealed that SuMV, SuCMoV and SuMMoV shared 99–100% identity within their groups which were distinct to other potyviruses. To best of our knowledge, this is the first detection, characterization and identification of viruses infecting sunflowers in Thailand.</p>

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Multiplex reverse transcription polymerase chain reaction for simultaneous detection and identification of multiple sunflower-infecting viruses

  • 期刊论文

  • 发表日期：

    2025年2月12日

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  • Salit Supakitthanakorn,
  • Kanungnit Reanwarakorn

摘要

Sunflowers are among the world’s most widely known oilseed and ornamental crops which can be infected by numerous viruses, leading to yield losses. This study surveyed and collected sunflower leaves showing virus-like symptoms such as mosaic, mottle, and chlorotic spots from different regions of Thailand during 2023–2024 to detect causative viruses. Three target viruses were investigated consisting of sunflower mosaic virus (SuMV) (proposed name Potyvirus helitessellati), sunflower mild mosaic virus (SuMMoV) (proposed name Potyvirus helitenuitessellati), and sunflower chlorotic mottle virus (SuCMoV) (proposed name Potyvirus helichloromaculae). A multiplex reverse transcription polymerase chain reaction (RT-PCR) assay was developed for simultaneous detection of mixed virus infections. The annealing temperature was optimized for multiplex RT-PCR amplification, along with sensitivity and specificity assays. A total of 190 sunflower samples from 22 growing areas in 12 provinces across 4 regions of Thailand were subjected to multiplex RT-PCR detection. Single infection of SuCMoV was most frequently detected, followed by SuMV and SuMMoV. Among mixed infections, SuMV and SuCMoV co-infection was the most common, and infections with all three viruses were also detected. Individual viruses were isolated and mechanically inoculated onto sunflowers and other plants to fulfill Koch’s postulates, revealing that all viruses caused distinct symptoms in sunflowers similar to field observations. Phylogenetic and sequence analysis revealed that SuMV, SuCMoV and SuMMoV shared 99–100% identity within their groups which were distinct to other potyviruses. To best of our knowledge, this is the first detection, characterization and identification of viruses infecting sunflowers in Thailand.