<p>Recently, a new scale was developed that assesses individual differences in AI-Interaction Positivity. This scale provides insight into degrees of well-being experienced when interacting with AI-systems (via items mostly tapping into affect). The original scale was validated in the German language, so the present study presents psychometric assessment for the scale’s English version with English-speaking participants. Two samples were investigated for this research question, where the first sample consisted of English native speakers (<i>n</i> = 302) and the second sample of competent—but non-English-native—speakers (<i>n</i> = 273). Results showed that greater AI-Interaction Positivity was associated with more positive attitudes towards AI in both samples as assessed with the acceptance dimension from the ATAI-scale and also a single item assessing positive attitudes towards AI. Inverse associations were observed with fearing AI and a single item for negative AI attitudes. The latter associations were weaker compared to the links between AI-Interaction Positivity and positive AI attitudes. Final analyses revealed mild positive associations between the primary emotional personality trait SEEKING and AI-Interaction Positivity in both samples. AI-Interaction Positivity mediated the relationship between SEEKING and positive AI attitudes. The present work shows that psychometric results for the English AI-well-being scale are sufficient and validation with existing AI attitude measures are meaningful.</p>