Scholarlink

<p>Aluminum oxide (Al<sub>2</sub>O<sub>3</sub>), often referred to as alumina, is widely studied for its notable properties, including high thermal stability, strong electrical insulation, large surface area, and biocompatibility. These characteristics have led to its use in many fields, ranging from environmental control to biomedical applications. Although alumina is commonly produced in various forms, such as beads, tubes, fibers, and powders, there are still limitations in its dynamic performance and interaction with the biological environment, particularly in laboratory applications. Several techniques that rely on bacterial cell disruption for the recovery of recombinant proteins at high yield and large scale have been developed. Among them, the use of alumina for cell lysis stands out as a particularly promising approach for extracting recombinant proteins from bacterial systems. However, only a limited number of studies have explored alumina-based lysis of bacterial cells cost-effectively and sustainably. This study stems from the need for a cost-effective, sustainable, and efficient approach for recombinant extraction-purification, especially in prokaryotic expression systems. Traditional cell disruption assays often require expensive reagents and equipment. This work proposes the use of aluminum oxide not only as a mechanical disruptor of bacterial cells but also as an adsorbent material for the purification of recombinant Vascular Endothelial Growth Factor (VEGF) proteins. Parameters such as temperature, pH, and the addition of organic compounds and detergents were optimized to enhance the release of recombinant proteins. Electrophoretic analysis and western blot confirmed that the combination of alumina with Imidazole and Sodium dodecyl sulfate (SDS) improved protein recovery with an increased yield of released protein after Imidazole treatment compared to SDS. The results indicate that alumina can be used as a low-cost, eco-friendly material for bacterial lysis and VEGF purification, providing a sustainable alternative for protein recovery.</p> Graphical abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Aluminum oxide as a clean support for the purification of recombinant vascular endothelial growth factor

  • 期刊论文

  • 发表日期：

    2026年6月18日

查看全文
  • Awatef Taktak,
  • Ali Gargouri

摘要

Aluminum oxide (Al2O3), often referred to as alumina, is widely studied for its notable properties, including high thermal stability, strong electrical insulation, large surface area, and biocompatibility. These characteristics have led to its use in many fields, ranging from environmental control to biomedical applications. Although alumina is commonly produced in various forms, such as beads, tubes, fibers, and powders, there are still limitations in its dynamic performance and interaction with the biological environment, particularly in laboratory applications. Several techniques that rely on bacterial cell disruption for the recovery of recombinant proteins at high yield and large scale have been developed. Among them, the use of alumina for cell lysis stands out as a particularly promising approach for extracting recombinant proteins from bacterial systems. However, only a limited number of studies have explored alumina-based lysis of bacterial cells cost-effectively and sustainably. This study stems from the need for a cost-effective, sustainable, and efficient approach for recombinant extraction-purification, especially in prokaryotic expression systems. Traditional cell disruption assays often require expensive reagents and equipment. This work proposes the use of aluminum oxide not only as a mechanical disruptor of bacterial cells but also as an adsorbent material for the purification of recombinant Vascular Endothelial Growth Factor (VEGF) proteins. Parameters such as temperature, pH, and the addition of organic compounds and detergents were optimized to enhance the release of recombinant proteins. Electrophoretic analysis and western blot confirmed that the combination of alumina with Imidazole and Sodium dodecyl sulfate (SDS) improved protein recovery with an increased yield of released protein after Imidazole treatment compared to SDS. The results indicate that alumina can be used as a low-cost, eco-friendly material for bacterial lysis and VEGF purification, providing a sustainable alternative for protein recovery.

Graphical abstract