<p>Purpose: Several studies have suggested that cortisol could be involved in type 2 diabetes (T2D) across different populations. However, this association has not been investigated in the Tunisian population. Our study assessed whether there is a link between T2D and morning blood cortisol levels in diabetic women from Tunisia. Subsequently, a comprehensive meta-analysis was carried out across blood, saliva, and urine samples. Methods: Thirty-three women from southern Tunisia were enrolled in this study. Multivariate logistic regression and receiver operating characteristic (ROC) curve analysis were performed to assess the cortisol-T2D association using R software. In addition, a meta-analysis was conducted using RevMan5.4 software. Besides, a comprehensive search of electronic databases (Google Scholar, PubMed, Scopus, and Circort) was performed. Seventy-three studies reporting baseline cortisol levels in patients diagnosed with T2D and healthy controls were included. The standardized mean difference in cortisol levels was calculated using Random effects model. Results: Morning plasma cortisol levels were significantly higher in T2D cases versus controls (<i>P</i> &lt; 0.001). Multivariate logistic regression, adjusted for age and BMI, confirmed this association (OR 1.021; 95% CI 1.009, 1.041; <i>P</i> = 0.007). ROC analysis showed moderate discriminative ability for cortisol (AUC 0.76; 95% CI [0.53, 0.98]), with an optimal cutoff of 390.3 nmol/L. The meta-analyses revealed significantly elevated blood and nocturnal salivary cortisol levels in diabetic patients compared to controls (SMD = 0.97; 95% CI [0.71, 1.22]; <InlineEquation ID="IEq1"> <EquationSource Format="TEX">\(P&lt;0.00001\)</EquationSource> </InlineEquation>; SMD = 1.4; 95% CI [0.76, 2.04]; <InlineEquation ID="IEq2"> <EquationSource Format="TEX">\(P&lt;0.0001\)</EquationSource> </InlineEquation>). Notably, T2D patients showed a blunted cortisol awakening response (CAR: SMD = -0.16; 95% CI [-0.29, -0.03]; <InlineEquation ID="IEq3"> <EquationSource Format="TEX">\(P=0.02\)</EquationSource> </InlineEquation>). Conclusion: The logistic regression and the meta-analysis revealed a significant association between high baseline cortisol levels and T2D across blood and saliva. These findings suggest the involvement of elevated baseline cortisol levels in T2D pathogenesis. However, this association needs further investigation.</p>

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The potential association between baseline cortisol levels and type 2 diabetes in Tunisian Women? a case-control study and meta-analysis across blood, saliva, and urine

  • Chiraz Zayani,
  • Imen Kallel,
  • Rania Abdelhedi,
  • Mouna Mnif Feki,
  • Faiza Fakhfakh

摘要

Purpose: Several studies have suggested that cortisol could be involved in type 2 diabetes (T2D) across different populations. However, this association has not been investigated in the Tunisian population. Our study assessed whether there is a link between T2D and morning blood cortisol levels in diabetic women from Tunisia. Subsequently, a comprehensive meta-analysis was carried out across blood, saliva, and urine samples. Methods: Thirty-three women from southern Tunisia were enrolled in this study. Multivariate logistic regression and receiver operating characteristic (ROC) curve analysis were performed to assess the cortisol-T2D association using R software. In addition, a meta-analysis was conducted using RevMan5.4 software. Besides, a comprehensive search of electronic databases (Google Scholar, PubMed, Scopus, and Circort) was performed. Seventy-three studies reporting baseline cortisol levels in patients diagnosed with T2D and healthy controls were included. The standardized mean difference in cortisol levels was calculated using Random effects model. Results: Morning plasma cortisol levels were significantly higher in T2D cases versus controls (P < 0.001). Multivariate logistic regression, adjusted for age and BMI, confirmed this association (OR 1.021; 95% CI 1.009, 1.041; P = 0.007). ROC analysis showed moderate discriminative ability for cortisol (AUC 0.76; 95% CI [0.53, 0.98]), with an optimal cutoff of 390.3 nmol/L. The meta-analyses revealed significantly elevated blood and nocturnal salivary cortisol levels in diabetic patients compared to controls (SMD = 0.97; 95% CI [0.71, 1.22]; \(P<0.00001\) ; SMD = 1.4; 95% CI [0.76, 2.04]; \(P<0.0001\) ). Notably, T2D patients showed a blunted cortisol awakening response (CAR: SMD = -0.16; 95% CI [-0.29, -0.03]; \(P=0.02\) ). Conclusion: The logistic regression and the meta-analysis revealed a significant association between high baseline cortisol levels and T2D across blood and saliva. These findings suggest the involvement of elevated baseline cortisol levels in T2D pathogenesis. However, this association needs further investigation.