<p>Agricultural production is considered one of the key factors that burden the environment because of the excessive use of machinery, energy, fossil oils, chemicals, and other necessary materials. In the present work the modern plant-growing method of aeroponics, a new innovative agricultural technique, is assessed for its mitigation potential in global warming. Specifically, the greenhouse gas emissions from aeroponic cucumber cultivation in an automatic greenhouse in Greece are assessed utilizing the life cycle assessment methodology. The functional unit was “1 Kg of fresh mass production of cucumbers”. The results showed that the total burden was approximately 2.06 total kg CO₂ eq.&#xa0;Our results indicate that electricity was the main source that contributes most to the environmental impact of aeroponic cucumber cultivation, due to its generation mix used. Compared with the results from other studies, the CO₂ eq.&#xa0;emissions per kg of cucumber (<i>Cucumis Sativus</i> L.) in the present work were higher than soil cultivation, and higher or lower, compared to other aeroponics, hydroponics and aquaponics studies, depending on the nature of cultivation system and plant species.</p>