Scholarlink

<p>Cerebral ischemia without reperfusion triggers extensive oxidative and metabolic disturbances with direct implications for environmental health and the biomedical valorization of natural bioactive compounds. This study investigates the neuroprotective effects of phycobiliproteins (PBP) extracted from <i>Spirulina platensis</i> a microalga of environmental and biotechnological interest in a Wistar rat model of unilateral carotid occlusion (UCCAO). Regional biochemical responses were assessed and compared with heparin and PBS controls. Rats were assigned to control, UCCAO untreated, PBS, heparin, and two PBP treatment groups. MDA, LDH, calcium, SOD, catalase, and GPx activities were measured in ipsilateral, contralateral hemispheres and cerebellum. PBP purity was confirmed spectrophotometrically (C-PC = 2.71, APC = 1.07, PE = 1.26). MDA levels increased significantly in untreated and PBS groups, whereas heparin and PBP dose 2 reversed oxidative damage. SOD and catalase activities, severely reduced under ischemia, were restored by treatment. GPx remained unchanged in cerebellar tissue. Calcium depletion and LDH dysfunction confirmed ischemic injury, with PBP dose 2 showing full recovery. Limited compensatory effect of the Circle of Willis and contralateral/cerebellar alterations indicated systemic propagation of ischemic stress. Overall, <i>Spirulina</i>-derived phycobiliproteins exhibited marked neuroprotective effects in non-reperfused cerebral and cerebellar ischemia by restoring antioxidant defenses, reducing oxidative damage, and normalizing metabolic markers. Their efficacy, particularly at 8&#xa0;mg/kgwas comparable to heparin and highlights their potential as environmentally derived phytotherapeutic agents contributing to eco-health and bioresource-based biomedical applications.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Protective effects of phycobiliproteins against cerebral and cerebellar ischemic damages in Wistar rat model

  • 期刊论文

  • 发表日期：

    2026年1月9日

查看全文
  • Adouani Basma,
  • Ferchichi Amal,
  • Zoghlami Manel,
  • Ben Attia Mossadok,
  • Khémiri Ikram

摘要

Cerebral ischemia without reperfusion triggers extensive oxidative and metabolic disturbances with direct implications for environmental health and the biomedical valorization of natural bioactive compounds. This study investigates the neuroprotective effects of phycobiliproteins (PBP) extracted from Spirulina platensis a microalga of environmental and biotechnological interest in a Wistar rat model of unilateral carotid occlusion (UCCAO). Regional biochemical responses were assessed and compared with heparin and PBS controls. Rats were assigned to control, UCCAO untreated, PBS, heparin, and two PBP treatment groups. MDA, LDH, calcium, SOD, catalase, and GPx activities were measured in ipsilateral, contralateral hemispheres and cerebellum. PBP purity was confirmed spectrophotometrically (C-PC = 2.71, APC = 1.07, PE = 1.26). MDA levels increased significantly in untreated and PBS groups, whereas heparin and PBP dose 2 reversed oxidative damage. SOD and catalase activities, severely reduced under ischemia, were restored by treatment. GPx remained unchanged in cerebellar tissue. Calcium depletion and LDH dysfunction confirmed ischemic injury, with PBP dose 2 showing full recovery. Limited compensatory effect of the Circle of Willis and contralateral/cerebellar alterations indicated systemic propagation of ischemic stress. Overall, Spirulina-derived phycobiliproteins exhibited marked neuroprotective effects in non-reperfused cerebral and cerebellar ischemia by restoring antioxidant defenses, reducing oxidative damage, and normalizing metabolic markers. Their efficacy, particularly at 8 mg/kgwas comparable to heparin and highlights their potential as environmentally derived phytotherapeutic agents contributing to eco-health and bioresource-based biomedical applications.