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<p>Seven cell disruption techniques—Manual Grinding (MG), Ultrasonication (US), Bead Beating (BB), French Press (FP), Enzymatic Treatment (ET), Freezing-Thawing (FT), and Three-Phase Partitioning (TPP)—were compared to extract antioxidant-rich biomolecules from <i>Tetraselmis chuii</i> and <i>Arthrospira platensis</i>. US (60&#xa0;min, 20&#xa0;kHz) emerged as the most effective method, yielding the highest amounts of released proteins : 589.52 ± 1.05&#xa0;mg/g for <i>A. platensis</i> and 335.58 ± 0.29&#xa0;mg/g for <i>T. chuii</i>. Carbohydrates, phenolic compounds, and pigments were more efficiently extracted with US. Microscopic observations confirmed extensive cell wall disruption under US, facilitating biomolecules release. Antioxidant assays demonstrated superior DPPH radical scavenging (92.64 ± 0.43%) and ferrous ion-chelating activity (85.58 ± 2.17%) for <i>T. chuii</i> extracts, whereas <i>A. platensis</i> exhibited optimal ferric reducing power (0.704 ± 0.003 at OD700nm). Essential amino-acid contents of protein-rich extracts, notably after US pretreatment, meet or exceed the FAO/WHO dietary requirements. Amino-acid profiling reveals that. <i>A. platensis</i> extracts are well-suited for functional foods targeting anabolic and metabolic performance, whereas <i>T. chuii</i> extracts may better support neurotransmitter biosynthesis, immune regulation, and nitrogen balance.</p>

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Comparison of disruption methods for obtaining antioxidant-rich extracts from Tetraselmis chuii and Arthrospira platensis

  • 期刊论文

  • 发表日期：

    2026年1月9日

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  • Montassar Romdhani,
  • Naima Nedjar,
  • Jihen Dhaouafi,
  • Anthony Massé,
  • Mourad Jridi,
  • Rafik Balti

摘要

Seven cell disruption techniques—Manual Grinding (MG), Ultrasonication (US), Bead Beating (BB), French Press (FP), Enzymatic Treatment (ET), Freezing-Thawing (FT), and Three-Phase Partitioning (TPP)—were compared to extract antioxidant-rich biomolecules from Tetraselmis chuii and Arthrospira platensis. US (60 min, 20 kHz) emerged as the most effective method, yielding the highest amounts of released proteins : 589.52 ± 1.05 mg/g for A. platensis and 335.58 ± 0.29 mg/g for T. chuii. Carbohydrates, phenolic compounds, and pigments were more efficiently extracted with US. Microscopic observations confirmed extensive cell wall disruption under US, facilitating biomolecules release. Antioxidant assays demonstrated superior DPPH radical scavenging (92.64 ± 0.43%) and ferrous ion-chelating activity (85.58 ± 2.17%) for T. chuii extracts, whereas A. platensis exhibited optimal ferric reducing power (0.704 ± 0.003 at OD700nm). Essential amino-acid contents of protein-rich extracts, notably after US pretreatment, meet or exceed the FAO/WHO dietary requirements. Amino-acid profiling reveals that. A. platensis extracts are well-suited for functional foods targeting anabolic and metabolic performance, whereas T. chuii extracts may better support neurotransmitter biosynthesis, immune regulation, and nitrogen balance.