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<p>This study investigates the efficacy of Halotolerant Plant Growth-Promoting Rhizobacteria in mitigating salinity constraint in durum wheat crop. The impact of bacterial inoculation was evaluated on the plant developmental parameters and the enzymatic activity of antioxidant system under salinity constraint. The response of three Tunisian durum wheat varieties Dhahbi, Nasr, and Salim to NaCl treatment at 100&#xa0;mM with and without halotolerant strains <i>Pseudomonas fluorescens</i> S13 and <i>Bacillus subtilis</i> S14 inoculation. For seedlings issued from non-inoculated seeds, salt stress significantly impacted the growth (plant height) and physiological parameters (leaf chlorophyll content) of all durum wheat varieties compared to the control, with varying intensity among the varieties. The lowest plant height reduction was exhibited with Dhahbi variety by 39%, while Nasr and Salim varieties showed higher and significant reductions by 49 and 65%, respectively as compared to controls (0&#xa0;mM NaCl). Similarly, a reduction in leaf chlorophyll content was observed, with a 35% decrease in the Nasr variety, and a more pronounced 55% decrease in the Salim variety. Interestingly, the salt effects were significantly reduced upon S13 and S14 bacterial strains inoculation, with varying effectiveness depending on the wheat variety. For Salim and Nasr, S13 and S14 increased SOD, catalase, and GPX activities in salt-treated plants, providing sufficient protection against intracellular oxidation and enhancing salinity tolerance compared to non-inoculated plants. Meanwhile, for the Dhahbi variety, inoculation with S13 or S14 strains didn’t show significant improvement in antioxidant enzyme activity in salt-treated plants. Nevertheless, there was a decrease in the production of H<sub>2</sub>O<sub>2</sub> and MDA relative to non-inoculated plants. The findings suggest that S13 and S14 strains effectively mitigate the impact of salinity on different durum wheat varieties, providing an eco-friendly approach to enhancing wheat crop yield under saline conditions. This research sheds light on novel strategies for sustainable agriculture by harnessing the benefits of rhizospheric bacteria on crop yield and stress tolerance.</p>

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Halotolerant rhizobacterial inoculation improves durum wheat phenotype under salinity constraint: a study on antioxidant enzymes and growth parameters

  • 期刊论文

  • 发表日期：

    2025年8月11日

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  • Imen Hemissi,
  • Hasna Ellouzi,
  • Amira Hachana,
  • Souhir Amraoui,
  • Rifka Hammami,
  • Hanen Arfaoui,
  • Amir Souissi,
  • Kamel Msaada,
  • Mohsen Hanana

摘要

This study investigates the efficacy of Halotolerant Plant Growth-Promoting Rhizobacteria in mitigating salinity constraint in durum wheat crop. The impact of bacterial inoculation was evaluated on the plant developmental parameters and the enzymatic activity of antioxidant system under salinity constraint. The response of three Tunisian durum wheat varieties Dhahbi, Nasr, and Salim to NaCl treatment at 100 mM with and without halotolerant strains Pseudomonas fluorescens S13 and Bacillus subtilis S14 inoculation. For seedlings issued from non-inoculated seeds, salt stress significantly impacted the growth (plant height) and physiological parameters (leaf chlorophyll content) of all durum wheat varieties compared to the control, with varying intensity among the varieties. The lowest plant height reduction was exhibited with Dhahbi variety by 39%, while Nasr and Salim varieties showed higher and significant reductions by 49 and 65%, respectively as compared to controls (0 mM NaCl). Similarly, a reduction in leaf chlorophyll content was observed, with a 35% decrease in the Nasr variety, and a more pronounced 55% decrease in the Salim variety. Interestingly, the salt effects were significantly reduced upon S13 and S14 bacterial strains inoculation, with varying effectiveness depending on the wheat variety. For Salim and Nasr, S13 and S14 increased SOD, catalase, and GPX activities in salt-treated plants, providing sufficient protection against intracellular oxidation and enhancing salinity tolerance compared to non-inoculated plants. Meanwhile, for the Dhahbi variety, inoculation with S13 or S14 strains didn’t show significant improvement in antioxidant enzyme activity in salt-treated plants. Nevertheless, there was a decrease in the production of H2O2 and MDA relative to non-inoculated plants. The findings suggest that S13 and S14 strains effectively mitigate the impact of salinity on different durum wheat varieties, providing an eco-friendly approach to enhancing wheat crop yield under saline conditions. This research sheds light on novel strategies for sustainable agriculture by harnessing the benefits of rhizospheric bacteria on crop yield and stress tolerance.