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<p>Daidzin (DZN) and phytol (PHY) are natural compounds reported to possess neuroprotective properties; however, their sedative potential remains unclear. This study evaluated their effects on thiopental sodium (TS)-induced sleeping behavior in male <i>Swiss</i> albino mice and explored possible receptor interactions using molecular docking analysis. Sixty mice were divided into ten groups (<i>n</i> = 6). Thirty minutes after oral pre-treatment with the test compounds or control, TS (20&#xa0;mg/kg, i.p.) was administered to induce sleep, and sleep latency and duration were recorded. Both DZN (2.5 and 5&#xa0;mg/kg) and PHY (25 and 50&#xa0;mg/kg) significantly reduced sleep latency and prolonged sleep duration compared to control (<i>p</i> &lt; 0.001). The combination of DZN-5 and PHY-50 further enhanced sleep duration (98.67 ± 2.70&#xa0;min) compared to individual treatments, while the triple combination (DZN-5 + PHY-50 + DZP-2) produced the longest sleep duration (120.17 ± 4.34&#xa0;min). Docking analysis showed binding affinities of − 8.0&#xa0;kcal/mol (DZN), − 5.5&#xa0;kcal/mol (PHY), and − 8.3&#xa0;kcal/mol (DZP) toward the GABA<sub>A</sub> receptor (α1 and β2 subunits). These findings indicate that DZN and PHY enhance TS-induced sleep and may involve GABAergic modulation, as suggested by in silico analysis. Further experimental studies are required to confirm receptor-level mechanisms.</p>

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Daidzin and Phytol Enhance the Sedative Effect of Diazepam and the Anesthetic Effects of Thiopental Sodium: In Vivo and Computational Assessments

  • 期刊论文

  • 发表日期：

    2026年4月29日

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  • Md. Torequl Islam,
  • Salehin Sheikh,
  • Raihan Chowdhury,
  • Md. Shimul Bhuia,
  • Md. Amirul Islam,
  • Isaac Moura Araújo,
  • Carolina Bandeira Domiciano,
  • Henrique Douglas Melo Coutinho,
  • Md. Saifuzzaman

摘要

Daidzin (DZN) and phytol (PHY) are natural compounds reported to possess neuroprotective properties; however, their sedative potential remains unclear. This study evaluated their effects on thiopental sodium (TS)-induced sleeping behavior in male Swiss albino mice and explored possible receptor interactions using molecular docking analysis. Sixty mice were divided into ten groups (n = 6). Thirty minutes after oral pre-treatment with the test compounds or control, TS (20 mg/kg, i.p.) was administered to induce sleep, and sleep latency and duration were recorded. Both DZN (2.5 and 5 mg/kg) and PHY (25 and 50 mg/kg) significantly reduced sleep latency and prolonged sleep duration compared to control (p < 0.001). The combination of DZN-5 and PHY-50 further enhanced sleep duration (98.67 ± 2.70 min) compared to individual treatments, while the triple combination (DZN-5 + PHY-50 + DZP-2) produced the longest sleep duration (120.17 ± 4.34 min). Docking analysis showed binding affinities of − 8.0 kcal/mol (DZN), − 5.5 kcal/mol (PHY), and − 8.3 kcal/mol (DZP) toward the GABAA receptor (α1 and β2 subunits). These findings indicate that DZN and PHY enhance TS-induced sleep and may involve GABAergic modulation, as suggested by in silico analysis. Further experimental studies are required to confirm receptor-level mechanisms.