<p>Given the wide variability in how biomaterials reduce carbon emissions—stemming from differences in both biomass feedstocks and the non-bio-based materials they replaced—this study employed a Life Cycle Assessment (LCA) to evaluate the potential of various biomaterials in mitigating greenhouse gas (GHG) emissions and contributing to reducing environmental impacts and cumulative energy demand. Three representative biomaterials: wood, bamboo, and kenaf fibers were examined across five case studies: wood replacing aluminum (Al) alloy, bamboo replacing polyvinyl chloride (PVC) pipes, bamboo replacing PVC packaging, natural kenaf fiber reinforced composites replacing glass fiber (GF) sheet molding compound (SMC), and wood replacing polycarbonate (PC). Their respective contributions to carbon neutrality were assessed using LCA. These materials individually impact environmental factors such as Global Warming Potential (GWP), acidification, etc. were identified throughout their lifecycle. Results indicated that the wood replacing Al alloy had the greatest impact on achieving carbon neutrality in terms of reducing GWP and cumulated energy demand (CED). An implicit analysis was conducted comparing GWP and CED in material substitution to highlight how different materials affect these environmental metrics and demonstrate the potential of alternative options in reducing GHG emissions and energy use across the lifecycle. It can be concluded that both the specific biomaterials employed and the non-bio-based materials they replace should be considered in order to effectively compare their respective contributions to carbon neutrality. This dual perspective is essential, as biomaterials differ in carbon sequestration and energy demand, while non-bio-based counterparts vary in emissions and energy intensity. Such insights can guide decision-makers in selecting effective strategies to optimize biomass utilization and advance carbon neutrality.</p>