High Cycle Fatigue (HCF) behaviour of hybrid SS316L steel specimens is experimentally investigated at room temperature. The tensile, microhardness, nanoindentation, and high cycle fatigue tests (at stress ratios \(R=0.1\) and \(R=-1\) ) are performed to examine the mechanical integrity and fatigue performance of the hybrid specimens. The hybrid specimen’s failure trend shows strong interface and excellent build strength of the AM part. Electron Backscattered Diffraction (EBSD) analysis reveals notable differences in microstructure between the AM and CM zones, specifically in grain size, kernel average misorientation (KAM), and dislocation densities. The post-fatigue analysis reveals low fractions of high-angle grain boundaries (HAGBs), reduced kernel average misorientation values and development of ideal texture in the AM zone, all of which contribute to its enhanced fatigue crack resistance. These findings underscore the synergistic mechanical performance achievable through hybrid additive conventional manufacturing strategies, contingent on process defect mitigation.