<p>The influence of layered casting process on the internal quality of large steel ingot was studied. Three furnaces of 500-kg rack steel ingots were prepared by traditional casting process and layered casting process, respectively. The microstructure of the ingots after pickling was observed. The content of elements in the central section of the ingots was measured by ICP-OES, and the segregation degree of elements in the ingots was analyzed. The experimental results show that the internal quality of layered casting ingot is significantly improved compared with that of conventional casting ingot, in which equiaxed crystal grains are significantly refined and the proportion is increased by more than 10%, and the low-power rating of ingot is improved by more than 0.5 grade. The segregation degree of each element in the ingot decreased significantly, and the segregation rate decreased by more than 20% on average. The segregation range of C element in the first furnace layered casting ingot decreased by 26.33% compared with that in conventional casting ingot. However, with the increase in casting interval, the segregation of elements in layered casting ingot deteriorates slightly, but it is still better than that in traditional casting ingot. The mechanism of restraining macrosegregation by layered casting process was analyzed.</p>