Background <p>Point of care testing is the key for screening cervical cancer. Cervical cancer is one of the most preventable cancers. The key factor to cervical cancer is the persistent infection of high – risk Human Papilloma Virus. The limiting factor to molecular point of care testing for screening for cervical cancer is the extraction of DNA.</p> Objective <p>This study is a pilot study in that direction. This study deals with the standardization of a buffer-based method for one step extraction of viral DNA from liquid-based cytology samples. The collection of the samples and processing is simplified and does not need specific equipment or trained staff or equipment.</p> Results <p>The best buffer contained MgCl2, EDTA, SDS, Triton × 100, PVP, CH3COOK and TE buffer and was evaluated with 260/280 ratios, single peak in spectophometer and Agarose gel electrophoresis. The samples were used in PCR and the results compared in an ROC curve with the conventional methods. The ROC curve value is 1, implying that one step method is comparable to the conventional methods.</p> Conclusion <p>This method aids in screening larger samples in limited resource setting. The lacuna in testing in limited resource setting like cost, time, sample processing can be overcome by this buffer. While studies in this area have been carried out. For tissues and viral DNA extraction this is the first of its kind.</p> Graphical Abstract <p></p>