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Purpose <p>This work assesses the synthesis of biocompatible nanoscaffolds containing carbon quantum dots (CQDs), as well as their physicochemical characteristics and biological effects on PC12 stem cells.</p> Methods <p>CQDs were synthesized via a microwave method using citric acid and dimethylamine, and then incorporated into PCL nanofibers through electrospinning. The scaffolds were characterized using DLS, FTIR, UV-visible spectra, PL, FE-SEM, and XRD. MTT assay, PC12 cell binding, and antioxidant activity were assessed via DPPH and H₂O₂ assays.</p> Results <p>Synthesized CQDs displayed uniform size, strong blue fluorescence, and characteristic functional groups. Incorporation of CQDs into PCL scaffolds significantly improved water uptake, degradation rate, and surface porosity. MTT assays confirmed excellent cytocompatibility and enhanced proliferation of PC12 cells. SEM imaging showed superior cell adhesion on PCL/CQD scaffolds. Antioxidant tests revealed high radical scavenging capacity and protective effects under oxidative stress. qRT-PCR showed no effect on Nanog gene expression.</p> Conclusion <p>PCL scaffolds enriched with CQDs showed favorable physicochemical and biological properties, including antioxidant and cytoprotective effects. CQDs enhanced hydrophilicity, degradation, and cell-scaffold interactions, without cytotoxic effects. These findings demonstrate the potential of PCL/CQD scaffolds in improving tissue engineering. Future studies should evaluate their long-term safety and in vivo performance.</p> Lay Summary <p>This study examined the use of carbon quantum dots (CQDs) in nanofibrous scaffolds for potential applications in tissue engineering. The engineered nanoscaffolds showed improved water uptake, faster degradation, and better surface properties that supported cell growth. PC12 cells attached well to the scaffolds and showed higher survival and growth rates. The CQDs also showed potent antioxidant effects that helped protect cells from oxidative damage. Importantly, the CQDs were not toxic to cells. Further studies are needed to confirm these findings and performance.</p>

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Carbon Quantum Dot-Enhanced Biodegradable Scaffolds: A Synergistic Strategy for Cellular Protection and Tissue Engineering

  • 期刊论文

  • 发表日期：

    2025年12月5日

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  • Faranak Hasanpour,
  • Saber Zahri,
  • Arash Abdolmaleki,
  • Asadollah Asadi

摘要

Purpose

This work assesses the synthesis of biocompatible nanoscaffolds containing carbon quantum dots (CQDs), as well as their physicochemical characteristics and biological effects on PC12 stem cells.

Methods

CQDs were synthesized via a microwave method using citric acid and dimethylamine, and then incorporated into PCL nanofibers through electrospinning. The scaffolds were characterized using DLS, FTIR, UV-visible spectra, PL, FE-SEM, and XRD. MTT assay, PC12 cell binding, and antioxidant activity were assessed via DPPH and H₂O₂ assays.

Results

Synthesized CQDs displayed uniform size, strong blue fluorescence, and characteristic functional groups. Incorporation of CQDs into PCL scaffolds significantly improved water uptake, degradation rate, and surface porosity. MTT assays confirmed excellent cytocompatibility and enhanced proliferation of PC12 cells. SEM imaging showed superior cell adhesion on PCL/CQD scaffolds. Antioxidant tests revealed high radical scavenging capacity and protective effects under oxidative stress. qRT-PCR showed no effect on Nanog gene expression.

Conclusion

PCL scaffolds enriched with CQDs showed favorable physicochemical and biological properties, including antioxidant and cytoprotective effects. CQDs enhanced hydrophilicity, degradation, and cell-scaffold interactions, without cytotoxic effects. These findings demonstrate the potential of PCL/CQD scaffolds in improving tissue engineering. Future studies should evaluate their long-term safety and in vivo performance.

Lay Summary

This study examined the use of carbon quantum dots (CQDs) in nanofibrous scaffolds for potential applications in tissue engineering. The engineered nanoscaffolds showed improved water uptake, faster degradation, and better surface properties that supported cell growth. PC12 cells attached well to the scaffolds and showed higher survival and growth rates. The CQDs also showed potent antioxidant effects that helped protect cells from oxidative damage. Importantly, the CQDs were not toxic to cells. Further studies are needed to confirm these findings and performance.