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<p><i>Ampelocissus latifolia (A. latifolia)</i>, a medicinal plant rich in bioactive compounds, was investigated for its potential application in bone tissue engineering. This study evaluated the antioxidant capacity, osteogenic potential, and safety profile of <i>A. latifolia</i> root extracts prepared with 70% hydroethanolic solution via Soxhlet extraction. Phytochemical analysis revealed high total phenolic content (TPC: 266.64 ± 9.01&#xa0;mg GAE/g) and total flavonoid content (TFC: 370.15 ± 24.76&#xa0;mg QE/g), suggesting potent antioxidative potential. Antioxidant activity assessed through 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and ferric reducing antioxidant power (FRAP) assays revealed EC₅₀ values of 18.60&#xa0;µg/mL, 11.02&#xa0;µg/mL, and 46.30&#xa0;µg/mL, respectively, compared with ascorbic acid (6.088, 4.67, and 4.388&#xa0;µg/mL). Gas chromatography–mass spectrometry (GC–MS) and ultrahigh-performance liquid chromatography–high-resolution mass spectrometry (UPLC-Q-TOF HRMS) profiling identified key bioactive compounds, including thymine, quinic acid, and n-hexadecanoic acid, which are known for their antioxidant and anti-inflammatory properties. In vitro studies demonstrated that the extract significantly enhanced cellular proliferation and osteogenic differentiation, as evidenced by increased alkaline phosphatase (ALP) activity and collagen synthesis, indicating early osteogenic commitment. Toxicological evaluations, including acute and subacute assessments in animal models, confirmed the safety and biocompatibility of the extract. No significant changes in body weight, organ function, or histopathology were observed, highlighting its nontoxic nature. These findings establish <i>A. latifolia</i> root extract as a promising natural biomaterial with osteoinductive and antioxidative properties, providing a basis for its application in bone regeneration and further preclinical development in tissue engineering strategies.</p>

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Exploring the Osteogenic Efficacy of Ampelocissus latifolia Root Extract as a Promising Phytotherapeutic Candidate for Tissue Engineering Applications

  • 期刊论文

  • 发表日期：

    2025年11月11日

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  • Geeta Kumari Wasupalli,
  • Dibya Ranjan Sahoo,
  • Srichandan Rath,
  • Reshma Pradhan,
  • Rabinarayan Acharya,
  • Pradeep Naik

摘要

Ampelocissus latifolia (A. latifolia), a medicinal plant rich in bioactive compounds, was investigated for its potential application in bone tissue engineering. This study evaluated the antioxidant capacity, osteogenic potential, and safety profile of A. latifolia root extracts prepared with 70% hydroethanolic solution via Soxhlet extraction. Phytochemical analysis revealed high total phenolic content (TPC: 266.64 ± 9.01 mg GAE/g) and total flavonoid content (TFC: 370.15 ± 24.76 mg QE/g), suggesting potent antioxidative potential. Antioxidant activity assessed through 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and ferric reducing antioxidant power (FRAP) assays revealed EC₅₀ values of 18.60 µg/mL, 11.02 µg/mL, and 46.30 µg/mL, respectively, compared with ascorbic acid (6.088, 4.67, and 4.388 µg/mL). Gas chromatography–mass spectrometry (GC–MS) and ultrahigh-performance liquid chromatography–high-resolution mass spectrometry (UPLC-Q-TOF HRMS) profiling identified key bioactive compounds, including thymine, quinic acid, and n-hexadecanoic acid, which are known for their antioxidant and anti-inflammatory properties. In vitro studies demonstrated that the extract significantly enhanced cellular proliferation and osteogenic differentiation, as evidenced by increased alkaline phosphatase (ALP) activity and collagen synthesis, indicating early osteogenic commitment. Toxicological evaluations, including acute and subacute assessments in animal models, confirmed the safety and biocompatibility of the extract. No significant changes in body weight, organ function, or histopathology were observed, highlighting its nontoxic nature. These findings establish A. latifolia root extract as a promising natural biomaterial with osteoinductive and antioxidative properties, providing a basis for its application in bone regeneration and further preclinical development in tissue engineering strategies.