Purpose Diabetic wounds represent a major health burden, and treatment involves skin grafting, tissue-engineered approaches, and cellular therapies. Skin grafting, though considered a golden standard, has limitations due to donor site morbidity. Cellular therapies are emerging as an alternate approach; however, they are restricted due to cellular migration and viability. Studies indicate that cellular functions occur via paracrine signalling factors rather than direct interactions. This finding has initiated the exploration of secretome-based therapy. Platelets and mesenchymal stem cells (MSCs) are two critical cell types involved in wound healing. MSCs and platelet interaction may affect the wound healing potential of the MSCs secretome. In the present study, we explored the effect of MSC secretome and platelet-induced MSC secretome on wound healing. Methods Adipose tissue-derived MSCs (ADMSCs) and platelet-rich plasma (PRP) were isolated from healthy rabbits (New Zealand White, 2 kg, 6 months) after obtaining Institutional Animal Ethics Committee clearance. Stem cell secretome and PRP-induced MSC secretome was prepared as per standard procedures. Hyperglycaemia was induced by growing fibroblasts in high-glucose (30 mM) Dulbecco’s Modified Eagle Medium (DMEM) with 5% foetal bovine serum (FBS) and antibiotics. The wound healing efficiency of PRP-induced MSC secretome and MSC secretome was evaluated by cell proliferation assay, migration assay, collagen synthesis analysis, reactive oxygen species (ROS) generation, actin cytoskeleton deposition, and gene expression analysis. Results PRP-induced MSC secretome treatment significantly increased cell proliferation and migration, improved collagen synthesis, reduced ROS generation, and promoted actin deposition in hyperglycaemic fibroblast cells compared to MSC secretome. Conclusion PRP-induced MSC secretome may be developed as an off-the-shelf therapeutic aid for diabetic wound healing. Further in vivo evaluation of PRP-induced MSC secretome needs to be performed in diabetic models, and the molecular mechanism of platelet-MSC interactions needs to be explored. Lay Summary Diabetic wounds affect millions of people worldwide leading to huge socio-economic health burden on patients. Therefore, more advanced, efficient, and easy-to-use treatment strategies need to be implanted. Platelets may induce MSCs and the altered cellular secretome may promote diabetic wound healing.

Wound Healing Effect of PRP-Induced MSC Secretome Under High Glucose Conditions: An In Vitro Study

期刊论文
发表日期：

2025年1月17日

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Reshma Murali,
Anugya Bhatt

摘要

Purpose

Diabetic wounds represent a major health burden, and treatment involves skin grafting, tissue-engineered approaches, and cellular therapies. Skin grafting, though considered a golden standard, has limitations due to donor site morbidity. Cellular therapies are emerging as an alternate approach; however, they are restricted due to cellular migration and viability. Studies indicate that cellular functions occur via paracrine signalling factors rather than direct interactions. This finding has initiated the exploration of secretome-based therapy. Platelets and mesenchymal stem cells (MSCs) are two critical cell types involved in wound healing. MSCs and platelet interaction may affect the wound healing potential of the MSCs secretome. In the present study, we explored the effect of MSC secretome and platelet-induced MSC secretome on wound healing.

Methods

Adipose tissue-derived MSCs (ADMSCs) and platelet-rich plasma (PRP) were isolated from healthy rabbits (New Zealand White, 2 kg, 6 months) after obtaining Institutional Animal Ethics Committee clearance. Stem cell secretome and PRP-induced MSC secretome was prepared as per standard procedures. Hyperglycaemia was induced by growing fibroblasts in high-glucose (30 mM) Dulbecco’s Modified Eagle Medium (DMEM) with 5% foetal bovine serum (FBS) and antibiotics. The wound healing efficiency of PRP-induced MSC secretome and MSC secretome was evaluated by cell proliferation assay, migration assay, collagen synthesis analysis, reactive oxygen species (ROS) generation, actin cytoskeleton deposition, and gene expression analysis.

Results

PRP-induced MSC secretome treatment significantly increased cell proliferation and migration, improved collagen synthesis, reduced ROS generation, and promoted actin deposition in hyperglycaemic fibroblast cells compared to MSC secretome.

Conclusion

PRP-induced MSC secretome may be developed as an off-the-shelf therapeutic aid for diabetic wound healing. Further in vivo evaluation of PRP-induced MSC secretome needs to be performed in diabetic models, and the molecular mechanism of platelet-MSC interactions needs to be explored.

Lay Summary

Diabetic wounds affect millions of people worldwide leading to huge socio-economic health burden on patients. Therefore, more advanced, efficient, and easy-to-use treatment strategies need to be implanted. Platelets may induce MSCs and the altered cellular secretome may promote diabetic wound healing.