Purpose <p>Tooth regeneration research seeks to revolutionize regenerative dentistry by integrating advanced tissue engineering and biomaterial strategies. This study emphasizes the development of innovative dental restoration techniques through the utilization of biomaterials that support cellular proliferation and differentiation, thereby offering viable alternatives to conventional dental methods.</p> Methods <p>This comprehensive review synthesizes current advancements in tissue engineering, focusing on the application of biomaterials such as smart materials, nanotechnology, and 3D-printed scaffolds. It also explores the role of induced pluripotent stem cells (iPSCs) and organoid cultures in enhancing tissue engineering approaches. Additionally, the review examines the integration of proteomics, phosphoproteomics, and genetic engineering systems in optimizing biomaterial interactions and tooth morphogenesis.</p> Results <p>Key findings highlight the transformative impact of biomaterials in tooth tissue engineering, including the development of scaffolds that mimic the natural extracellular matrix and promote neurovascular integration. The use of iPSCs and organoid cultures has further advanced the field by enabling precise modeling of tooth development and enhancing the differentiation of dental tissues. Moreover, innovative computational analyses have identified novel epithelial support tissues, contributing to more effective dental tissue differentiation.</p> Conclusion <p>This review provides a comprehensive overview of tissue engineering and biomaterial advancements in tooth regeneration. By integrating cutting-edge techniques and innovative biomaterials, regenerative dentistry is poised to achieve significant breakthroughs, offering promising avenues for future advancements in tooth regeneration.</p> Future Works <p>Future research will concentrate on refining bioengineered tooth prototypes for clinical application, enhancing the integration of biomaterials, and advancing genomic editing to perfect tooth regeneration. Efforts will aim to transition from theoretical frameworks to practical, patient-specific solutions, revolutionizing the treatment and management of dental ailments with cutting-edge science.</p> Lay Summary <p>Exploring breakthroughs in dental medicine, this review focuses on tissue engineering and biomaterials. Advanced techniques such as 3D printing and nanotechnology are pivotal in developing scaffolds that mimic natural tooth structures. The potential of these technologies to transform dental treatments is examined, offering insights into creating bioengineered teeth that could replace traditional dental restorations. The integration of innovative biomaterials and computational analysis opens new avenues for personalized regenerative dentistry, enhancing the effectiveness and customization of future dental care.</p>