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<p>The fungal pathogen <i>Ceratocystis manginecans</i> M. van Wyk, Al-Adawi &amp; M.J. Wingf. causes serious diseases in numerous plant species globally. This study examines the molecular functions and biological processes potentially linked to virulence and host adaptation in <i>C. manginecans</i> pathogenesis. During their interaction with <i>A. mangium</i>, two <i>C. manginecans</i> isolates, FRIM1162 (host: <i>A. mangium</i>) (CAM) and FRIM1441 (host: <i>Eucalyptus pellita</i>) (CEP), were profiled and observed for protein expression. After inoculation, 1-hour and 24-hour protein expressions&#xa0;were examined. A TMT-labelled protein profile from four groups showed 205 proteins, providing insights into the molecular pathogenesis. Proteomics data are available via ProteomeXchange with identifier PXD066081. Temporal protein expression patterns differed between isolates, suggesting varying cellular responses to host defence. Early infection stages involved proteins associated with DNA repair and cellular respiration. Host-specific adaptability appeared to involve reprogramming of metabolism and modulation of cellular transport. The genomic and functional diversity observed suggests specialisation in host-pathogen interactions. Additionally, putative protein-protein interaction networks were computationally inferred, highlighting predicted clusters of proteins involved in antioxidant defence, energy metabolism, and other core biological processes. While these predicted interactions suggest possible mechanisms underlying pathogen survival and virulence, they require further experimental validation. These insights may aid in identifying potential targets for disease control strategies.</p>

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Profiling pathogenesis stages of Ceratocystis manginecans infecting the stem of Acacia mangium using Tandem Mass Tag (TMT) quantitative proteomics

  • 期刊论文

  • 发表日期：

    2025年12月9日

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  • Samsuddin Ahmad Syazwan,
  • Mansor Patahayah,
  • Muhammad Syahmi Hishamuddin,
  • Rozi Mohamed

摘要

The fungal pathogen Ceratocystis manginecans M. van Wyk, Al-Adawi & M.J. Wingf. causes serious diseases in numerous plant species globally. This study examines the molecular functions and biological processes potentially linked to virulence and host adaptation in C. manginecans pathogenesis. During their interaction with A. mangium, two C. manginecans isolates, FRIM1162 (host: A. mangium) (CAM) and FRIM1441 (host: Eucalyptus pellita) (CEP), were profiled and observed for protein expression. After inoculation, 1-hour and 24-hour protein expressions were examined. A TMT-labelled protein profile from four groups showed 205 proteins, providing insights into the molecular pathogenesis. Proteomics data are available via ProteomeXchange with identifier PXD066081. Temporal protein expression patterns differed between isolates, suggesting varying cellular responses to host defence. Early infection stages involved proteins associated with DNA repair and cellular respiration. Host-specific adaptability appeared to involve reprogramming of metabolism and modulation of cellular transport. The genomic and functional diversity observed suggests specialisation in host-pathogen interactions. Additionally, putative protein-protein interaction networks were computationally inferred, highlighting predicted clusters of proteins involved in antioxidant defence, energy metabolism, and other core biological processes. While these predicted interactions suggest possible mechanisms underlying pathogen survival and virulence, they require further experimental validation. These insights may aid in identifying potential targets for disease control strategies.