Scholarlink

<p>Foot rot disease, caused by <i>Phytophthora capsici</i>, often leads to mortality in black pepper (<i>Piper nigrum</i> L.), though some plants show field tolerance. Plant microbiome can influence host resistance and/or pathogen virulence. Bacteria associated with healthy vines of <i>Piper nigrum</i> from diseased fields, and <i>Piper colubrinum</i>, a wild, foot rot disease resistant species, were explored for suppression of <i>Phytophthora</i> induced foliar infection in nursery plantlets of black pepper. After isolation of bacteria, <i>in vitro</i> screening for antagonism against the pathogen by dual culture plate assay and detached leaf assay was done. Additionally, in a cut-stem assay, the isolates were further screened for disease suppression, ensuring that the pathogen and antagonists are kept spatially apart. This novel assay is proven to be a rapid mode for selecting bacterial antagonists that induce systemic resistance against <i>P. capsici</i>. In a greenhouse experiment, the lowest disease index was observed in plants treated with the&#xa0;isolates <i>Bacillus velezensis</i> PCRS 5 and <i>Bacillus</i> sp. PNRO 6, when challenged with the pathogen, which resulted in 78 % and 77 % disease suppression respectively. The isolate <i>Bacillus</i> sp. PNRO 6 consistently exhibited superior foot rot suppression across all screening methods and excelled in the <i>in vivo</i> experiment. Bacterial treatment also enhanced systemic resistance in black pepper by promoting the production of defence-related enzymes. Treatment of stem cuttings, the planting material of black pepper, with defence-inducing bacterial strains may be a practical approach for the management of <i>Phytophthora</i> foliar infection.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Defence-inducing biocontrol bacteria associated with Piper spp. suppress foliar infection of black pepper by Phytophthora capsici

  • 期刊论文

  • 发表日期：

    2025年11月5日

查看全文
  • R. Abarna,
  • K. N. Anith

摘要

Foot rot disease, caused by Phytophthora capsici, often leads to mortality in black pepper (Piper nigrum L.), though some plants show field tolerance. Plant microbiome can influence host resistance and/or pathogen virulence. Bacteria associated with healthy vines of Piper nigrum from diseased fields, and Piper colubrinum, a wild, foot rot disease resistant species, were explored for suppression of Phytophthora induced foliar infection in nursery plantlets of black pepper. After isolation of bacteria, in vitro screening for antagonism against the pathogen by dual culture plate assay and detached leaf assay was done. Additionally, in a cut-stem assay, the isolates were further screened for disease suppression, ensuring that the pathogen and antagonists are kept spatially apart. This novel assay is proven to be a rapid mode for selecting bacterial antagonists that induce systemic resistance against P. capsici. In a greenhouse experiment, the lowest disease index was observed in plants treated with the isolates Bacillus velezensis PCRS 5 and Bacillus sp. PNRO 6, when challenged with the pathogen, which resulted in 78 % and 77 % disease suppression respectively. The isolate Bacillus sp. PNRO 6 consistently exhibited superior foot rot suppression across all screening methods and excelled in the in vivo experiment. Bacterial treatment also enhanced systemic resistance in black pepper by promoting the production of defence-related enzymes. Treatment of stem cuttings, the planting material of black pepper, with defence-inducing bacterial strains may be a practical approach for the management of Phytophthora foliar infection.