<p>Endospore-forming bacteria were obtained from the soil of tobacco fields in Cuba. The growth inhibition effect of these bacteria was tested against 13 phytopathogenic strains of <i>Nocardia</i> spp. causing ‘false Broomrape’ in tobacco plants. The test was based on dual culture and agar diffusion assays. As a result, seven isolates were found to inhibit the growth of most <i>Nocardia</i> spp. strains tested in this study. Of these, the A5 and A9 isolates were the most promising due to their high antagonistic effect; it must be mentioned that these were the only gamma hemolytic isolates. The phenotypical characterization and partial 16S rRNA gene sequencing identified these isolates as members of the genus <i>Bacillus</i>, phylogenetically related to <i>B. subtilis</i> and <i>B. halotolerans</i>, respectively. Two applications of a bioproduct based on an aqueous suspension of the A5 antagonist, one immediately after transplanting and another after the hilling work, allow greater control over the manifestation of tobacco ‘false Broomrape’ disease in the tobacco cultivar, ‘Corojo 2006’, under field conditions. To the best of our knowledge, this is the first report of antagonistic bacteria against phytopathogenic <i>Nocardia</i> spp. The A5 and A9 isolates are promising agents in the biological control of tobacco ‘false Broomrape’ disease.</p>