Scholarlink

<p><i>Fusarium</i> root rot (FRR) of beans causes considerable losses in the production of common bean and cowpea, which are important staple foods in Brazil. <i>Fusarium solani</i> f. sp. <i>phaseoli</i> has been attributed as the etiological agent of this disease, but under the current understanding of&#xa0;the phylogenetic species concept, this <i>forma specialis</i> may represent different species. In this study, we propose to identify species of the <i>Fusarium solani</i> species complex – FSSC associated with FRR on common bean and cowpea based on molecular phylogenetic analyses and test its pathogenicity. A set of 66 strains was obtained from symptomatic plants collected in distinct geographical regions of Brazil. Based on the phylogenetic analyses of partial DNA sequences of <i>RPB2</i>, we identified <i>F. paranaense</i> (<i>n</i> = 44), <i>F. solani</i> sensu stricto (<i>n</i> = 8), <i>F. suttonianum</i> (<i>n</i> = 10), and <i>F. martii</i> (<i>n </i>= 2), all members of FSSC Clade 3. Two strains were identified as <i>F. brasiliense</i>, a member of Clade 2, according to analyses of <i>RPB2</i>, IGS and locus 44 regions. In pathogenicity tests on common bean and cowpea, all species induced similar symptoms, such as rotting from the main root extending to the hypocotyl and reduction of plant growth. To confirm that these fungi cause disease also in soybean, we inoculated soybean plants. There, <i>F. brasiliense</i>, <i>F. paranaense</i>, <i>F. solani</i> s. str<i>.</i>, <i>F. suttonianum</i>, and <i>F. martii</i> induced root lesions. <i>Fusarium brasiliense</i> was the most virulent on all hosts in comparison with other FSSC species. With this study, we clarify the etiology of FRR in Brazil based on phylogenetic analysis and pathogenicity, complementing the concept of <i>forma specialis</i>. Additionally, we provide relevant support to disease management and plant breeding studies such as the selection of cultivars with resistance to <i>Fusarium</i> bean root rot in the field.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Members of the Fusarium solani species complex causing root rot of common bean and cowpea in Brazil

  • 期刊论文

  • 发表日期：

    2025年2月18日

查看全文
  • Janaina M. Sousa,
  • Marileide M. Costa,
  • Gláucia M. Moreira,
  • Arianna S. Menezes,
  • Cristiano S. Lima,
  • Ludwig H. Pfenning

摘要

Fusarium root rot (FRR) of beans causes considerable losses in the production of common bean and cowpea, which are important staple foods in Brazil. Fusarium solani f. sp. phaseoli has been attributed as the etiological agent of this disease, but under the current understanding of the phylogenetic species concept, this forma specialis may represent different species. In this study, we propose to identify species of the Fusarium solani species complex – FSSC associated with FRR on common bean and cowpea based on molecular phylogenetic analyses and test its pathogenicity. A set of 66 strains was obtained from symptomatic plants collected in distinct geographical regions of Brazil. Based on the phylogenetic analyses of partial DNA sequences of RPB2, we identified F. paranaense (n = 44), F. solani sensu stricto (n = 8), F. suttonianum (n = 10), and F. martii (n = 2), all members of FSSC Clade 3. Two strains were identified as F. brasiliense, a member of Clade 2, according to analyses of RPB2, IGS and locus 44 regions. In pathogenicity tests on common bean and cowpea, all species induced similar symptoms, such as rotting from the main root extending to the hypocotyl and reduction of plant growth. To confirm that these fungi cause disease also in soybean, we inoculated soybean plants. There, F. brasiliense, F. paranaense, F. solani s. str., F. suttonianum, and F. martii induced root lesions. Fusarium brasiliense was the most virulent on all hosts in comparison with other FSSC species. With this study, we clarify the etiology of FRR in Brazil based on phylogenetic analysis and pathogenicity, complementing the concept of forma specialis. Additionally, we provide relevant support to disease management and plant breeding studies such as the selection of cultivars with resistance to Fusarium bean root rot in the field.