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Purpose <p>In vivo imaging of adult zebrafish remains technically challenging due to loss of optical transparency and the lack of standardized molecular imaging protocols. The development of reliable radiotracer delivery and acquisition parameters is essential for improving imaging reproducibility and translational relevance.</p> Methods <p>Adult zebrafish with hepatobiliary tumor models were imaged using an integrated PET/MR system following intracardiac injection of F-18 FDG. Animals were assigned to four groups based primarily on uptake time, ranging from approximately 40 to 90&#xa0;min, with injected activity maintained within a narrow range. A standardized imaging workflow, including anesthesia, positioning, PET acquisition, and MRI co-registration, was implemented. Image quality, tracer distribution, and tumor visualization were qualitatively compared across groups.</p> Results <p>Intracardiac injection provided reliable systemic tracer delivery across all groups. Short uptake times resulted in high background activity, particularly in the gastrointestinal tract, while prolonged uptake times led to signal decay. An intermediate uptake time (~ 60&#xa0;min) appeared to provide the most favorable balance between signal intensity and background suppression under the current experimental setting. MRI consistently delineated hepatic lesions, while PET signal quality varied depending on protocol parameters. Semi-quantitative analysis indicated higher tracer uptake in lesion regions relative to adjacent background tissue.</p> Conclusion <p>This study establishes the first feasible PET/MR imaging workflow and identifies preliminary imaging conditions for adult zebrafish, demonstrating that uptake time is a critical determinant of imaging quality. The proposed workflow provides a reproducible platform for longitudinal in vivo imaging and may serve as a practical intermediate platform for preclinical molecular imaging prior to rodent studies.</p>

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Feasibility of a PET/MR Imaging Workflow in Adult Zebrafish Using Intracardiac FDG Injection

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年7月25日

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  • Chi-Yu Lai,
  • Avery Landon Wang,
  • Chi-Han Wu,
  • Guor-Mour Her,
  • Skye Hsin-Hsien Yeh,
  • Wen-Tao Huang,
  • Yuh-Feng Wang

摘要

Purpose

In vivo imaging of adult zebrafish remains technically challenging due to loss of optical transparency and the lack of standardized molecular imaging protocols. The development of reliable radiotracer delivery and acquisition parameters is essential for improving imaging reproducibility and translational relevance.

Methods

Adult zebrafish with hepatobiliary tumor models were imaged using an integrated PET/MR system following intracardiac injection of F-18 FDG. Animals were assigned to four groups based primarily on uptake time, ranging from approximately 40 to 90 min, with injected activity maintained within a narrow range. A standardized imaging workflow, including anesthesia, positioning, PET acquisition, and MRI co-registration, was implemented. Image quality, tracer distribution, and tumor visualization were qualitatively compared across groups.

Results

Intracardiac injection provided reliable systemic tracer delivery across all groups. Short uptake times resulted in high background activity, particularly in the gastrointestinal tract, while prolonged uptake times led to signal decay. An intermediate uptake time (~ 60 min) appeared to provide the most favorable balance between signal intensity and background suppression under the current experimental setting. MRI consistently delineated hepatic lesions, while PET signal quality varied depending on protocol parameters. Semi-quantitative analysis indicated higher tracer uptake in lesion regions relative to adjacent background tissue.

Conclusion

This study establishes the first feasible PET/MR imaging workflow and identifies preliminary imaging conditions for adult zebrafish, demonstrating that uptake time is a critical determinant of imaging quality. The proposed workflow provides a reproducible platform for longitudinal in vivo imaging and may serve as a practical intermediate platform for preclinical molecular imaging prior to rodent studies.