<p>The increasing demand for environmentally sustainable materials has intensified interest in natural fibers as alternatives to synthetic reinforcements. In this study, fibers were extracted from the stems of <i>Phragmites australis</i> using a simple pond-based water retting technique, and their structural, biological, and functional properties were systematically evaluated. XRD analysis confirmed a highly ordered cellulose I lattice with an exceptionally high crystallinity index of 95%, while FTIR spectra verified the presence of cellulose, hemicellulose, and lignin. Notably, the fibers exhibited strong antibacterial activity against <i>Escherichia coli</i>, producing inhibition zones of 23&#xa0;mm and 28&#xa0;mm at (LC - 50&#xa0;µg &amp; HC - 100&#xa0;µg), comparable to the efficacy of streptomycin 29&#xa0;mm (10&#xa0;µg). Confocal laser scanning microscopy further revealed significant antibiofilm activity, highlighting their relevance for bioactive applications. Mechanical testing demonstrated a tensile strength of 13.62&#xa0;MPa, with samples exhibiting brittle failure behavior consistent with their high crystallinity. Thermogravimetric analysis showed that the fibers remained thermally stable up to 300&#xa0;°C and yielded nearly 10% char residue at 500&#xa0;°C, indicating satisfactory thermal endurance. Collectively, these findings demonstrate that <i>Phragmites australis</i> fibers possess well-defined structural ordering, potent antibacterial and antibiofilm properties, and moderate mechanical and thermal stability. These attributes position them as potential sustainable materials for bioactive and engineering composite applications, offering performance advantages while reducing reliance on environmentally detrimental synthetic fibers.</p>

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Green Extraction and Characterization of Phragmites australis Stem Fibers: Unlocking Their Potential for Sustainable Bioactive Applications

  • Jay Bhushan Jawale,
  • T. Raja,
  • G. Dhanraj,
  • K. B. Prabhalakshmi

摘要

The increasing demand for environmentally sustainable materials has intensified interest in natural fibers as alternatives to synthetic reinforcements. In this study, fibers were extracted from the stems of Phragmites australis using a simple pond-based water retting technique, and their structural, biological, and functional properties were systematically evaluated. XRD analysis confirmed a highly ordered cellulose I lattice with an exceptionally high crystallinity index of 95%, while FTIR spectra verified the presence of cellulose, hemicellulose, and lignin. Notably, the fibers exhibited strong antibacterial activity against Escherichia coli, producing inhibition zones of 23 mm and 28 mm at (LC - 50 µg & HC - 100 µg), comparable to the efficacy of streptomycin 29 mm (10 µg). Confocal laser scanning microscopy further revealed significant antibiofilm activity, highlighting their relevance for bioactive applications. Mechanical testing demonstrated a tensile strength of 13.62 MPa, with samples exhibiting brittle failure behavior consistent with their high crystallinity. Thermogravimetric analysis showed that the fibers remained thermally stable up to 300 °C and yielded nearly 10% char residue at 500 °C, indicating satisfactory thermal endurance. Collectively, these findings demonstrate that Phragmites australis fibers possess well-defined structural ordering, potent antibacterial and antibiofilm properties, and moderate mechanical and thermal stability. These attributes position them as potential sustainable materials for bioactive and engineering composite applications, offering performance advantages while reducing reliance on environmentally detrimental synthetic fibers.