Influence of Pulse Energy on Synthesis of Trimetallic Nanoparticles Via Pulsed Laser Ablation in Liquid for Enhanced Surface Plasmon Resonance and Bioactivity
摘要
Controlling the pulsed laser ablation parameters in liquids is crucial for determining the optical and structural properties of nanoparticles and their application in the medical field. This research aimed to investigate the effect of pulse energy on the shape and size of trimetallic nanoparticles synthesized by pulsed laser ablation in deionized water. Two different laser pulse energies, 500 mJ and 1500 mJ, were used on metallic targets at a constant ablation duration of 10 min. Scanning electron microscopy analysis revealed that increasing the pulse energy from 500 mJ to 1500 mJ resulted in a decrease in the average particle size from 55 nm to 24 nm. X-ray diffraction analysis confirmed the successful synthesis of gold, silver, and palladium nanoparticles within the nanostructures. While UV-Vis spectroscopy revealed a significant blue shift at the surface plasmon resonance peak, 524 nm at 500 mJ and 465 nm at 1500 mJ. Consequently, the sample prepared with an ablation energy of 1500 mJ exhibited antibacterial activity against E. coli and S. aureus, with inhibition zones reaching 23–24 mm compared to 19–22 mm for the sample ablation at 500 mJ. These results indicate that increasing the pulse energy to 1500 mJ resulted in a controlled increase in the size of the nanoparticles, which greatly enhanced the surface plasmon resonance and biological activity against bacteria compared to the nanoparticles at 500 mJ.