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<p>In this study, silver nanoparticles (Ag NPs) were synthesized using the pulsed laser ablation (PLA) technique at varying laser energies (200, 300, 400, and 500 mJ) with a constant pulse duration of 100 ns. The physicochemical properties of the nanoparticles were characterized using X-ray diffraction (XRD), scanning electron microscopy (SEM), and atomic force microscopy (AFM). XRD analysis confirmed that the Ag NPs exhibit a face-centered cubic (FCC) crystalline structure. A noticeable decrease in the energy band gap was observed with increasing laser energies from 200 to 500 mJ. SEM images revealed well-dispersed clusters, with most nanoparticles exhibiting a spherical shape, alongside a few with cubic-like morphology. AFM results showed that increasing the laser energies led to larger grain sizes, increased surface roughness, and higher root mean square (RMS) values. The antibacterial assessment demonstrated that Ag NPs are highly effective against both Gram-positive and Gram-negative bacteria, particularly at lower laser energies (200, 300, and 400 mJ). This strong antimicrobial effect is attributed to the electrostatic interaction between the nanoparticle surfaces and bacterial cell membranes. Overall, the study confirms that Ag NPs synthesized via PLA possess significant antibacterial activity and hold promise for use in combating harmful microorganisms.</p>

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Evaluation of Antibacterial Effects of Silver Nanoparticles Synthesized via Pulsed Laser Ablation at Different Laser Energies

  • 期刊论文

  • 发表日期：

    2025年5月9日

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  • Nagham M. Hamid,
  • Hiba A. Abed,
  • Othman A. Fahad

摘要

In this study, silver nanoparticles (Ag NPs) were synthesized using the pulsed laser ablation (PLA) technique at varying laser energies (200, 300, 400, and 500 mJ) with a constant pulse duration of 100 ns. The physicochemical properties of the nanoparticles were characterized using X-ray diffraction (XRD), scanning electron microscopy (SEM), and atomic force microscopy (AFM). XRD analysis confirmed that the Ag NPs exhibit a face-centered cubic (FCC) crystalline structure. A noticeable decrease in the energy band gap was observed with increasing laser energies from 200 to 500 mJ. SEM images revealed well-dispersed clusters, with most nanoparticles exhibiting a spherical shape, alongside a few with cubic-like morphology. AFM results showed that increasing the laser energies led to larger grain sizes, increased surface roughness, and higher root mean square (RMS) values. The antibacterial assessment demonstrated that Ag NPs are highly effective against both Gram-positive and Gram-negative bacteria, particularly at lower laser energies (200, 300, and 400 mJ). This strong antimicrobial effect is attributed to the electrostatic interaction between the nanoparticle surfaces and bacterial cell membranes. Overall, the study confirms that Ag NPs synthesized via PLA possess significant antibacterial activity and hold promise for use in combating harmful microorganisms.