Scholarlink

<p>Urdbean (<i>Vigna mungo</i> L Hepper), is an Indian-origin, self-pollinated and nutritionally important Kharif pulse cultivated in various parts of country. Its productivity is relatively low due to narrow genetic base and limited research on molecular aspects. Therefore, during the present study, genetic diversity was evaluated using Simple Sequence Repeat (SSR) markers among 40 superior urdbean genotypes, selected from a set of 96 genotypes, based on 2&#xa0;years (<i>Kharif</i> 2023–2024) of field screening at Soybean Farm, College of Agriculture, Jawaharlal Nehru Krishi Vishwa Vidyalaya (JNKVV), Jabalpur, Madhya Pradesh, India. These genotypes were analyzed using 58 SSR markers, of which 41 produced clear amplification, including 22 polymorphic and 19 monomorphic markers. The polymorphic markers exhibited Polymorphic Information Content (PIC) values ranging from 0.24 to 0.97, with markers Cp05325 and LR738A identified as the most informative. Major allelic frequency ranged from 0.48 to 0.97, while the average gene diversity was estimated at 0.18. The Marker Index (MI) ranged from 0.09 to 0.90, Resolving Power (RP) from 0.04 to 0.52, Discriminating Power (DP) from 0.04 to 3.79, and Heterozygosity Index (HI) from 0.04 to 4.01. Cluster analysis based on the Unweighted Pair Group Method with Arithmetic mean (UPGMA) classified the genotypes into two major clusters, with genotype KUG1179 forming a distinct and genetically divergent group. In addition, heatmap analysis based on thirteen agronomic traits revealed substantial phenotypic variation among the evaluated genotypes. The findings demonstrate the effectiveness of SSR markers in detecting genetic variability and elucidating genetic relationships among urdbean genotypes. These results provide valuable insights for germplasm characterization, parental selection, and the development of improved urdbean varieties through breeding programs.</p> Graphical abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Microsatellite markers-based diversity assessment in urdbean genotypes (Vigna mungo L Hepper)

  • 期刊论文

  • 发表日期：

    2026年7月29日

查看全文
  • Ayushi Soni,
  • Stuti Sharma,
  • Shikha Upadhyay,
  • R. S. Sharma,
  • R. Shiv Ramakrishnan

摘要

Urdbean (Vigna mungo L Hepper), is an Indian-origin, self-pollinated and nutritionally important Kharif pulse cultivated in various parts of country. Its productivity is relatively low due to narrow genetic base and limited research on molecular aspects. Therefore, during the present study, genetic diversity was evaluated using Simple Sequence Repeat (SSR) markers among 40 superior urdbean genotypes, selected from a set of 96 genotypes, based on 2 years (Kharif 2023–2024) of field screening at Soybean Farm, College of Agriculture, Jawaharlal Nehru Krishi Vishwa Vidyalaya (JNKVV), Jabalpur, Madhya Pradesh, India. These genotypes were analyzed using 58 SSR markers, of which 41 produced clear amplification, including 22 polymorphic and 19 monomorphic markers. The polymorphic markers exhibited Polymorphic Information Content (PIC) values ranging from 0.24 to 0.97, with markers Cp05325 and LR738A identified as the most informative. Major allelic frequency ranged from 0.48 to 0.97, while the average gene diversity was estimated at 0.18. The Marker Index (MI) ranged from 0.09 to 0.90, Resolving Power (RP) from 0.04 to 0.52, Discriminating Power (DP) from 0.04 to 3.79, and Heterozygosity Index (HI) from 0.04 to 4.01. Cluster analysis based on the Unweighted Pair Group Method with Arithmetic mean (UPGMA) classified the genotypes into two major clusters, with genotype KUG1179 forming a distinct and genetically divergent group. In addition, heatmap analysis based on thirteen agronomic traits revealed substantial phenotypic variation among the evaluated genotypes. The findings demonstrate the effectiveness of SSR markers in detecting genetic variability and elucidating genetic relationships among urdbean genotypes. These results provide valuable insights for germplasm characterization, parental selection, and the development of improved urdbean varieties through breeding programs.

Graphical abstract