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<p><?tk 4?>In orchids, protocorm-like bodies (PLBs) hold significant potential as an alternative to conventional micropropagation techniques. Mass propagation of moth orchids (<i>Phalaenopsis amabilis</i>) via PLBs, is a routine method in commercial floriculture. Plant growth regulators (PGRs) plays important roles in the induction and development of PLBs. The present study performed as factorial in a completely randomized design with two factors, Thidiazuron (0, 0.5, 1.0, and 1.5&#xa0;mg/L) and 6-benzylaminopurine (0, 1.0, 2.0, and 3.0&#xa0;mg/L), with the objective of producing PLBs in <i>Phalaenopsis amabilis</i> cv. Anthura Beijing. In the second phase, the regeneration and growth rate of plantlets derived from PLBs in a PGR-free culture medium were investigated. The results revealed that the age and type of explants and the combination of 6-benzylaminopurine (6-BAP) and Thidiazuron (TDZ) significantly affected PLB formation. Increasing the BAP concentration reduced both the fresh and dry weights, number, and diameter of the PLBs. High concentrations of TDZ (1.5&#xa0;mg/L) combined with low concentrations of 6-BAP (1.0&#xa0;mg/L) led to the highest PLB number and plantlet production. In the plantlet regeneration and rooting phase, plantlets obtained from PLBs derived from media with low concentrations of TDZ and 6-BAP presented the best growth parameters. The combination of PGRs in the PLB production phase also affects the regeneration and rooting of plantlets in PGR-free medium. The highest root number, and fresh and dry weight of roots observed in plantlets derived from that PLBs produced in a medium with lowrer concentrations of BAP and TDZ. Results revealed that only the basal part of shoot tip leaves was regenerative.</p> Graphical abstract <p></p>

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Thidiazuron, explant type and age affect the PLB regeneration of Phalaenopsis orchid

  • 期刊论文

  • 发表日期：

    2026年2月18日

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  • Khadijeh Aghaei,
  • Parviz Noruzi,
  • Javad Rezapour Fard

摘要

In orchids, protocorm-like bodies (PLBs) hold significant potential as an alternative to conventional micropropagation techniques. Mass propagation of moth orchids (Phalaenopsis amabilis) via PLBs, is a routine method in commercial floriculture. Plant growth regulators (PGRs) plays important roles in the induction and development of PLBs. The present study performed as factorial in a completely randomized design with two factors, Thidiazuron (0, 0.5, 1.0, and 1.5 mg/L) and 6-benzylaminopurine (0, 1.0, 2.0, and 3.0 mg/L), with the objective of producing PLBs in Phalaenopsis amabilis cv. Anthura Beijing. In the second phase, the regeneration and growth rate of plantlets derived from PLBs in a PGR-free culture medium were investigated. The results revealed that the age and type of explants and the combination of 6-benzylaminopurine (6-BAP) and Thidiazuron (TDZ) significantly affected PLB formation. Increasing the BAP concentration reduced both the fresh and dry weights, number, and diameter of the PLBs. High concentrations of TDZ (1.5 mg/L) combined with low concentrations of 6-BAP (1.0 mg/L) led to the highest PLB number and plantlet production. In the plantlet regeneration and rooting phase, plantlets obtained from PLBs derived from media with low concentrations of TDZ and 6-BAP presented the best growth parameters. The combination of PGRs in the PLB production phase also affects the regeneration and rooting of plantlets in PGR-free medium. The highest root number, and fresh and dry weight of roots observed in plantlets derived from that PLBs produced in a medium with lowrer concentrations of BAP and TDZ. Results revealed that only the basal part of shoot tip leaves was regenerative.

Graphical abstract