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<p>Sweet potato is vegetatively propagated, vines produced from previous crop cycle are planting material for new crop cycle, producing phytosanitary risks, due to diseases dispersion that affect crop yield and quality. In vitro micropropagation includes cleaning processes that ensure phytosanitary quality. Further, this efficient massive production of indexed in vitro plants is standardized by optimal growth conditions which includes specific growth regulators in the culture medium. These initial stages of seed systems for vegetatively propagated species are critical for ensuring the production of high-quality planting material for distribution as certified seed in commercial cultivation. This study aimed to optimize the culture medium of in vitro initiation and propagation stages of sweet potato through supplementation by 1-naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP), to improve the growth and efficiency in propagation rate of indexed vitroplants. These regulators were incorporated, both individually and in combination, at two concentrations (0.2 and 0.5&#xa0;mg·L<sup>− 1</sup>). Growth parameters, indices, and rates were evaluated as response variables. The results indicated that in vitro sweet potato seedlings cultivated under 0.2&#xa0;mg·L<sup>− 1</sup> of NAA presented a balanced biomass distribution, generating significantly major nodes number (increase up to 100% and 45% at initiation and propagation stages, respectively) and plant height (increase up to 80% and 25% at initiation and propagation stages, respectively) than control, which are traits related to major propagation rates. Contrary, higher concentration of NAA (0.5&#xa0;mg·L<sup>− 1</sup>) in combination with BAP limited plant growth and development, presenting low values in growth indexes and accumulation of biomass in non-photosynthetic structures (callus). Exogenous supplementation adding NAA at certain concentration, into initiation and propagation medium could be incorporated to improve propagation rate of high-quality planting material at initial steps of sweet potato seed system.</p>

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Improving micropropagation of orange-fleshed sweet potato (Ipomoea batatas) using 1-naphthaleneacetic acid and 6-benzylaminopurine

  • 期刊论文

  • 发表日期：

    2025年12月29日

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  • Jazmín Vanessa Pérez-Pazos,
  • Rocío Gámez,
  • Amparo Rosero

摘要

Sweet potato is vegetatively propagated, vines produced from previous crop cycle are planting material for new crop cycle, producing phytosanitary risks, due to diseases dispersion that affect crop yield and quality. In vitro micropropagation includes cleaning processes that ensure phytosanitary quality. Further, this efficient massive production of indexed in vitro plants is standardized by optimal growth conditions which includes specific growth regulators in the culture medium. These initial stages of seed systems for vegetatively propagated species are critical for ensuring the production of high-quality planting material for distribution as certified seed in commercial cultivation. This study aimed to optimize the culture medium of in vitro initiation and propagation stages of sweet potato through supplementation by 1-naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP), to improve the growth and efficiency in propagation rate of indexed vitroplants. These regulators were incorporated, both individually and in combination, at two concentrations (0.2 and 0.5 mg·L− 1). Growth parameters, indices, and rates were evaluated as response variables. The results indicated that in vitro sweet potato seedlings cultivated under 0.2 mg·L− 1 of NAA presented a balanced biomass distribution, generating significantly major nodes number (increase up to 100% and 45% at initiation and propagation stages, respectively) and plant height (increase up to 80% and 25% at initiation and propagation stages, respectively) than control, which are traits related to major propagation rates. Contrary, higher concentration of NAA (0.5 mg·L− 1) in combination with BAP limited plant growth and development, presenting low values in growth indexes and accumulation of biomass in non-photosynthetic structures (callus). Exogenous supplementation adding NAA at certain concentration, into initiation and propagation medium could be incorporated to improve propagation rate of high-quality planting material at initial steps of sweet potato seed system.