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<p>Mineral nutrients play an important role in plant growth, development, stress resilience, and overall yield. Among major and minor mineral nutrients, silicon (Si) is a non-essential but especially beneficial mineral nutrient that plays a critical role in cereal crops by enhancing defense response and mitigating abiotic stresses. The genetic and molecular mechanism of Si is well studied in rice; however, its role at the single-cell level remains elusive in sorghum and other cereal crops. Additionally, how Si treatment modulates the leaf transcriptome at the single-cell level remains unclear. Using single-nucleus RNA sequencing (snRNA-seq), we profiled the cellular and transcriptional responses of sorghum leaves to Si supplementation. Si-treated leaves showed substantial Si accumulation, primarily in specialized silica cells. Transcriptomic analysis identified 16 distinct cell clusters and revealed that Si treatment upregulated genes involved in stress-related metabolic pathways and transport processes. Importantly, we uncovered a cell-specific regulation of Si transporters, with differential expression in xylem and epidermal cells. These findings advance our understanding of nutrient regulation at the single-cell level and provide a foundation for enhancing nutrient efficiency and stress tolerance in sorghum.</p>

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Single-cell transcriptomic landscape of sorghum leaf reveals cell-specific mineral nutrient regulation

  • 期刊论文

  • 发表日期：

    2025年11月12日

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  • Leonidas D’Agostino,
  • Vikas Devkar,
  • Yong-Villalobos Lenin,
  • S. V. Krishna Jagadish,
  • Rupesh Deshmukh,
  • Luis Herrera-Estrella,
  • Gunvant B. Patil

摘要

Mineral nutrients play an important role in plant growth, development, stress resilience, and overall yield. Among major and minor mineral nutrients, silicon (Si) is a non-essential but especially beneficial mineral nutrient that plays a critical role in cereal crops by enhancing defense response and mitigating abiotic stresses. The genetic and molecular mechanism of Si is well studied in rice; however, its role at the single-cell level remains elusive in sorghum and other cereal crops. Additionally, how Si treatment modulates the leaf transcriptome at the single-cell level remains unclear. Using single-nucleus RNA sequencing (snRNA-seq), we profiled the cellular and transcriptional responses of sorghum leaves to Si supplementation. Si-treated leaves showed substantial Si accumulation, primarily in specialized silica cells. Transcriptomic analysis identified 16 distinct cell clusters and revealed that Si treatment upregulated genes involved in stress-related metabolic pathways and transport processes. Importantly, we uncovered a cell-specific regulation of Si transporters, with differential expression in xylem and epidermal cells. These findings advance our understanding of nutrient regulation at the single-cell level and provide a foundation for enhancing nutrient efficiency and stress tolerance in sorghum.