<p>Wood, a versatile and renewable material, has potential for various structural applications. The emergence of engineered wood products, such as Glued-Laminated Timber (GLT) or Glulam, has expanded the use of reforestation woods as structural elements due to the possibility of selection or removal of defects. The estimation of the bending stiffness (EI) of the structural element made of GLT, among other properties, becomes complex due to the variation of the modulus of elasticity of the lamellas, a problem that has motivated the use of various analytical models for the estimation of EI. Thus, after the manufacture and characterization of eucalyptus GLT beams glued with resorcinol formaldehyde adhesive, the objective of this research was to apply various analytical methods, the formulation of ABNT NBR 7190–1:2022/ASTM D3737:2018 and also the use of numerical simulation via the finite element method (FEM), in the estimation of bending stiffness, making it possible to identify the method or calculation approach of greater precision. The results indicate that the analytical models evaluated, including the normative model, are statistically equivalent to each other, showing slightly lower accuracy compared to the numerical model that accounts for wood orthotropy. Nevertheless, they are suitable for estimating the bending stiffness of GLT beams in structural design applications.</p>