<p>Astereae, comprising approximately 252 genera and 3100 species, is the second largest tribe within the family Asteraceae. Members of this tribe are most commonly found in temperate regions. Astereae is considered the sister tribe of Anthemideae. The taxonomy and subtribal classification of the Astereae are challenging and controversial. Consequently, pollen morphology is a practical and reliable tool for studying the systematics of the Astereae due to its conserved features. This study aims to identify and describe the pollen types within Astereae based on pollen features and to investigate the relationships among subtribes, genera and species using these data. The pollen micromorphology of 39 specimens representing 11 genera of Astereae was examined using scanning electron microscopy. Clustering and ordination methods identified three pollen types—<i>Aster</i>, <i>Bellis</i> and <i>Symphyotrichum</i>—among the studied taxa. PCA analysis revealed that the distance between spines was the most important pollen feature for distinguishing the three groups. Therefore, pollen morphology played a reliable role in the delimitation of the studied species within the tribe and identifying the different pollen types. Using the pollen data, the taxonomic problems related to the delimitation between the genera <i>Aster</i> and <i>Erigeron</i> were resolved based on differences in pollen micromorphology. The studied Astereae taxa demonstrated the greatest similarity in pollen features with the tribe Anthemideae, particularly in features such as exine ornamentation and spine size.</p>