<p><i>Bactris mexicana</i> Mart. is a native palm species distributed in the subperennial rainforests of southeastern Mexico, represents a potentially valuable source of lignocellulosic biomass for sustainable applications. This study aimed to determine the lignocellulosic composition of the stipe at different heights (basal, middle, and apical regions) and to evaluate its structural variation to assess its potential for bioproduct development. Chemical extractions were performed to quantify extractives, cellulose, hemicellulose, and lignin content. Spectroscopic analyses (FTIR) and epifluorescence microscopy were applied to characterize cellulose crystallinity and lignin composition. The results indicated that total extractive content ranged from 26.25 to 28.59%, with no significant variation along the stipe. Cellulose, hemicellulose, and lignin percentages were also statistically homogeneous across the three regions, although the basal region had slightly higher lignin content, while the apical section presented greater cellulose content. FTIR spectra revealed consistent chemical structures, and crystallinity indices suggested a predominance of type I crystalline cellulose. Lignin analysis showed a dominance of Guaiacyl units, with the Syringyl/Guaiacyl (S/G) ratio varying slightly by region. Microscopy confirmed the even distribution of lignocellulosic compounds and revealed tannins embedded in the parenchyma. The overall chemical and structural homogeneity of the stipe suggests that any section of <i>B. mexicana</i> could be effectively used as a lignocellulosic raw material. These findings expand current knowledge of neotropical palms and support the potential of this species as a renewable source for biofuel production and fiber-based applications, while emphasizing the need for future studies on its mechanical properties and resistance to degradation.</p>