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<p>The rhizosphere bacteria play crucial roles in plant health and growth as they are involved in assimilating nutrients and resisting adverse conditions such as nutrient stress, drought, and wind erosion. <i>Agriophyllum squarrosum</i> (L.) Moq. is a pioneer plant used in sand fixation due to its strong resistance to drought and wind erosion. However, the bacterial community characteristics and ecological function in the rhizosphere of <i>A. squarrosum</i> are poorly understood. In this study, soil samples were collected from different developmental stages (seedling stage, vegetative stage, reproductive stage, and withering stage) of <i>A. squarrosum</i>. Illumina Miseq sequencing was used to detect differences in soil bacterial abundance. The Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) program was used to predict bacterial functions, and the relationships among bacteria, functional populations, and soil nutrients were examined using a heatmap analysis. The results showed that the Shannon and Sobs indices of rhizosphere bacteria were significantly higher during the reproductive stage than during the other stages. <i>Pantoea</i> sp. (7.03%) was the dominant genus during the seedling stage; <i>Arthrobacter</i> sp. was the dominant genus during the vegetative (13.94%), reproductive (7.57%), and withering (12.30%) stages. The relative abundances of Chloroflexi, Acidobacteria, and Gemmatimonadetes were significantly high during the reproductive stage. According to the PICRUSt analysis, membrane transport, signal transduction, and environmental adaptation of the bacterial functional population occurred during the seedling stage. Carbohydrate metabolism increased during the vegetative stage, while energy metabolism, lipid metabolism, and biosynthesis of other secondary metabolites of the bacterial functional population significantly increased during the reproductive stage. The abundances of bacterial communities, functional genes, and soil nutrients were synergistically altered during various developmental stages. Our findings suggest that the developmental stages of <i>A. squarrosum</i> play a significant role in defining the composition and structure of bacterial communities in the rhizosphere. The results will provide a basis for better prediction and understanding of soil bacterial metabolic potential and functions of <i>A. squarrosum</i> rhizosphere in sandy areas.</p>

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Rhizosphere bacterial communities of Agriophyllum squarrosum (L.) Moq. during different developmental stages

  • 期刊论文

  • 发表日期：

    2025年9月30日

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  • Shengnan Zhang,
  • Haiyan Gao,
  • Shanshan Yang,
  • Lei Zhang,
  • Deren Yan,
  • Haiguang Huang,
  • Zhiguo Yang,
  • Junwen Li,
  • Yuekun Tang,
  • Hongbin Xu

摘要

The rhizosphere bacteria play crucial roles in plant health and growth as they are involved in assimilating nutrients and resisting adverse conditions such as nutrient stress, drought, and wind erosion. Agriophyllum squarrosum (L.) Moq. is a pioneer plant used in sand fixation due to its strong resistance to drought and wind erosion. However, the bacterial community characteristics and ecological function in the rhizosphere of A. squarrosum are poorly understood. In this study, soil samples were collected from different developmental stages (seedling stage, vegetative stage, reproductive stage, and withering stage) of A. squarrosum. Illumina Miseq sequencing was used to detect differences in soil bacterial abundance. The Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) program was used to predict bacterial functions, and the relationships among bacteria, functional populations, and soil nutrients were examined using a heatmap analysis. The results showed that the Shannon and Sobs indices of rhizosphere bacteria were significantly higher during the reproductive stage than during the other stages. Pantoea sp. (7.03%) was the dominant genus during the seedling stage; Arthrobacter sp. was the dominant genus during the vegetative (13.94%), reproductive (7.57%), and withering (12.30%) stages. The relative abundances of Chloroflexi, Acidobacteria, and Gemmatimonadetes were significantly high during the reproductive stage. According to the PICRUSt analysis, membrane transport, signal transduction, and environmental adaptation of the bacterial functional population occurred during the seedling stage. Carbohydrate metabolism increased during the vegetative stage, while energy metabolism, lipid metabolism, and biosynthesis of other secondary metabolites of the bacterial functional population significantly increased during the reproductive stage. The abundances of bacterial communities, functional genes, and soil nutrients were synergistically altered during various developmental stages. Our findings suggest that the developmental stages of A. squarrosum play a significant role in defining the composition and structure of bacterial communities in the rhizosphere. The results will provide a basis for better prediction and understanding of soil bacterial metabolic potential and functions of A. squarrosum rhizosphere in sandy areas.