Unpredictable spontaneous stress study of Olea europaea ethanolic leaf extract based on qualitative analysis, in silico and animal model
摘要
The worldwide illnesses are stress and disorders associated with stressors. This research work checks the anti-stress capability of Olea europaea leaf extract in ethanol as an in vivo and in silico anti-stress agent. Molecular docking and ADMET studies show that most plant components have excellent intermolecular interactions with protein receptors and show drug-like properties. The LD50 of Olea europaea is safe up to 1000 mg/kg body weight per oral (p.o.) weight found in acute oral toxicity. As a result, we selected 400 and 600 mg/kg as the two dosages for anti-stress activity in the current investigation. Antistress activity: when compared to normal and unpredictable spontaneous stress groups in a dose-based manner, the results show a corresponding decline in MDA and MPO levels and a rise in GSH, CAT, and SOD levels when stress models containing Olea europaea leaf extract and Geriforte Syrup are used in the unpredictable spontaneous stress model. The histological analysis shows significant recovery from cardiac, stomach, and brain injuries. Improved tissue architecture and decreased inflammatory and degenerative alterations demonstrated the dose-dependent (400 & 600 mg/kg) cardioprotective, gastroprotective, and neuroprotective benefits of treatment with OE ethanolic leaf extract. The brain, stomach, and heart tissues healed nearly normally at the higher dosage. These protective effects were similar to those seen with conventional Geriforte therapy. Because Olea europaea leaf extract contains phenolic components, specifically iridoids and secoiridoids, as well as antioxidant molecules including ligstroside, oleuropein, hydroxytyrosol, tyrosol, and caffeic acid, the results showed the potential anti-stress action of the extract.