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Background <p>Gestational diabetes mellitus (GDM) is a metabolic disorder characterized by glucose intolerance that develops during pregnancy. Recent studies have begun to elucidate the role of MicroRNAs (miRNAs) in the pathophysiology of GDM, suggesting that these non-coding RNAs could serve as potential biomarkers for the condition. This study aimed to investigate the clinical diagnostic value of miR-520g-3p in GDM patients.</p> Methods <p>GSE104297 dataset was downloaded and analyzed by R software to obtain the differentially expressed miRNAs. A total of 110 GDM patients and 110 healthy pregnant women were recruited and the serum samples were collected. The expressions of miRNAs in the serum samples were detected by qRT-PCR assay. Then, the diagnostic value of miR-520g-3p in GDM patients were analyzed by receiver operating curve (ROC) analysis. The correlation between miR-520g-3p expression and patient clinical information was analyzed using Pearson analysis.</p> Results <p>Through the GSE104297 dataset, we obtained a total of 15 upregulated and 15 downregulated miRNAs in the feto-placental endothelial cells of GDM patients. Then, in the serum of GDM patients, miR-520g-3p, miR-519d-3p, miR-145-3p, miR-1246 and miR-671-3p expression levels were significantly increased, while miR-579-3p and miR-378a-5p were decreased. Among them, the change in miR-520g-3p is the most significant. Furthermore, the results of ROC analysis showed that the AUCs of miR-520g-3p in GDM patients from normal pregnant women were 0.861. miR-520g-3p levels was significantly positively correlated with FBG, OGTT (1&#xa0;h and 2&#xa0;h) and TG.</p> Conclusion <p>This study identifies miR-520g-3p might be a promising biomarker for GDM, demonstrating significant correlations with clinical characteristics and diagnostic potential.</p>

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Expression and clinical significance of mir-520g-3p in patients with gestational diabetes mellitus

  • 期刊论文

  • 发表日期：

    2025年5月22日

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  • Yi Ding,
  • Min Cai,
  • Yan Wang,
  • Runpeng Zhao,
  • Xin Yue

摘要

Background

Gestational diabetes mellitus (GDM) is a metabolic disorder characterized by glucose intolerance that develops during pregnancy. Recent studies have begun to elucidate the role of MicroRNAs (miRNAs) in the pathophysiology of GDM, suggesting that these non-coding RNAs could serve as potential biomarkers for the condition. This study aimed to investigate the clinical diagnostic value of miR-520g-3p in GDM patients.

Methods

GSE104297 dataset was downloaded and analyzed by R software to obtain the differentially expressed miRNAs. A total of 110 GDM patients and 110 healthy pregnant women were recruited and the serum samples were collected. The expressions of miRNAs in the serum samples were detected by qRT-PCR assay. Then, the diagnostic value of miR-520g-3p in GDM patients were analyzed by receiver operating curve (ROC) analysis. The correlation between miR-520g-3p expression and patient clinical information was analyzed using Pearson analysis.

Results

Through the GSE104297 dataset, we obtained a total of 15 upregulated and 15 downregulated miRNAs in the feto-placental endothelial cells of GDM patients. Then, in the serum of GDM patients, miR-520g-3p, miR-519d-3p, miR-145-3p, miR-1246 and miR-671-3p expression levels were significantly increased, while miR-579-3p and miR-378a-5p were decreased. Among them, the change in miR-520g-3p is the most significant. Furthermore, the results of ROC analysis showed that the AUCs of miR-520g-3p in GDM patients from normal pregnant women were 0.861. miR-520g-3p levels was significantly positively correlated with FBG, OGTT (1 h and 2 h) and TG.

Conclusion

This study identifies miR-520g-3p might be a promising biomarker for GDM, demonstrating significant correlations with clinical characteristics and diagnostic potential.