Scholarlink

Objectives <p>To evaluate the effects of a very-low-calorie diet (VLCD) on insulin resistance (IR), metabolic gene expression, and fatty acid profiles in obese patients (body mass index [BMI] ≥ 30&#xa0;kg/m<sup>2</sup>) undergoing bariatric surgery compared to age- and sex-matched nonobese controls (BMI ≤ 25&#xa0;kg/m<sup>2</sup>) undergoing elective abdominal surgery.</p> Methods <p>A total of 38 participants (21 obese and 17 nonobese controls) were recruited for this study. Obese patients underwent VLCD (800&#xa0;kcal/day) for four weeks before surgery. Fasting blood samples and tissue biopsies were collected during surgery. Key parameters included IR (measured using HOMA-IR), metabolic gene expression (quantified via RT-PCR), and fatty acid composition (analyzed by gas chromatography). Data were compared between pre- and post-VLCD groups in the obese cohort.</p> Results <p>GLUT4 expression was reduced (1.57-fold, <i>p</i> = 0.025), whereas PDK4 (3.9-fold, <i>p</i> = 0.002), CPT1 (2.5-fold, <i>p</i> = 0.013), and AMPK (twofold, <i>p</i> = 0.004) expression were Correlation analysis revealed that GLUT4 was negatively correlated with BMI (r = -0.85), glucose (r = -0.94), and IR (r = -0.79), CPT1 was positively correlated with these parameters (BMI: r = 0.84, glucose: r = 0.92, IR: r = 0.82). VLCD significantly reduced monounsaturated fatty acids, including alpha-linolenic acid (<i>p</i> = 0.03) and erucic acid (<i>p</i> = 0.019). Postsurgical improvements included reductions in BMI (Δ = 6.21, <i>p</i> &lt; 0.0001), glucose level (Δ = 6.94, <i>p</i> = 0.0007), and IR (Δ = 10.19, <i>p</i> = 0.0039).</p> Conclusion <p>VLCD modulated metabolic gene expression and fatty acid profiles, enhancing IR and metabolic health both pre- and post-surgery. This represents a critical strategy for optimizing the outcomes of obese patients undergoing bariatric surgery.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Regulation of metabolic pathways genes and the effects of very low-calorie diet on insulin resistance and fatty acid profiles in obese patients undergoing bariatric surgery

  • 期刊论文

  • 发表日期：

    2025年5月7日

查看全文
  • Manoj Kumar,
  • Vibhav Nigam,
  • Sandeep Kumar,
  • Anumesh K. Pathak

摘要

Objectives

To evaluate the effects of a very-low-calorie diet (VLCD) on insulin resistance (IR), metabolic gene expression, and fatty acid profiles in obese patients (body mass index [BMI] ≥ 30 kg/m2) undergoing bariatric surgery compared to age- and sex-matched nonobese controls (BMI ≤ 25 kg/m2) undergoing elective abdominal surgery.

Methods

A total of 38 participants (21 obese and 17 nonobese controls) were recruited for this study. Obese patients underwent VLCD (800 kcal/day) for four weeks before surgery. Fasting blood samples and tissue biopsies were collected during surgery. Key parameters included IR (measured using HOMA-IR), metabolic gene expression (quantified via RT-PCR), and fatty acid composition (analyzed by gas chromatography). Data were compared between pre- and post-VLCD groups in the obese cohort.

Results

GLUT4 expression was reduced (1.57-fold, p = 0.025), whereas PDK4 (3.9-fold, p = 0.002), CPT1 (2.5-fold, p = 0.013), and AMPK (twofold, p = 0.004) expression were Correlation analysis revealed that GLUT4 was negatively correlated with BMI (r = -0.85), glucose (r = -0.94), and IR (r = -0.79), CPT1 was positively correlated with these parameters (BMI: r = 0.84, glucose: r = 0.92, IR: r = 0.82). VLCD significantly reduced monounsaturated fatty acids, including alpha-linolenic acid (p = 0.03) and erucic acid (p = 0.019). Postsurgical improvements included reductions in BMI (Δ = 6.21, p < 0.0001), glucose level (Δ = 6.94, p = 0.0007), and IR (Δ = 10.19, p = 0.0039).

Conclusion

VLCD modulated metabolic gene expression and fatty acid profiles, enhancing IR and metabolic health both pre- and post-surgery. This represents a critical strategy for optimizing the outcomes of obese patients undergoing bariatric surgery.