Objectives <p>Sarcopenic obesity is a multifactorial disorder commonly found in elderly individuals. One contributing factor is gut microbiota dysbiosis. This study compared the abundance of certain bacteria in elderly individuals with obesity and sarcopenic obesity.</p> Methods <p>The study included 50 elderly individuals over 65 with a body mass index (BMI) of over 30&#xa0;kg/m², both sexes. Participants were divided into two groups, each with 25 individuals, based on the diagnosis of sarcopenia using the EWGSOP2 criteria. Individuals with underlying diseases, those using antibiotics, and those with a history of gastrointestinal surgery were excluded. Stool samples were stored at -80&#xa0;°C, and DNA was extracted using standard kits. Bacterial DNA sample quality was assessed using a Nanodrop device. Bacterial frequency was measured using qPCR. The log cfu for each bacteria was calculated and compared in both groups using an independent t-test. Spearman measured the correlation between bacterial genera and physical performance in SPSS 26.</p> Results <p>The case group had a significantly higher average age (70.96) than the control group (68.32). The average BMI was the same in both groups. The frequency of <i>Escherichia</i> (p-value = 0.046) and <i>Bifidobacterium</i> (p-value = 0.017) was significantly higher in the case group. There was no significant difference in the frequency of <i>Lactobacillus</i> and <i>Akkermansia</i>.</p> Conclusion <p>The study uncovered substantial differences in gut microbiota composition between elderly individuals experiencing sarcopenic obesity and those with obesity alone. The findings suggest that dysbiosis, characterized by an excessive presence of <i>Bifidobacterium</i>, <i>Escherichi</i>a, and <i>Akkermansia</i>, may be associated with sarcopenic obesity.</p>