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Background <p>Endometriosis is a gynecological disorder that affects a large number of women worldwide, with several complications including chronic pelvic pain, infertility, and reduced health. Considering the <i>MAPK</i> signaling pathway is involved in endometriosis, we examined the hsa-miR-199a-5p and its predicted target gene, <i>MAP3K11</i>, expression levels to investigate an association between their concurrent dysregulation and endometriosis and to assess their expression changes in peripheral blood mononuclear cells (PBMCs).</p> Methods <p>The sequences of hsa-miR-199a-5p and its target gene, <i>MAP3K11</i>, were obtained by using TargetScan and miRDB databases. Blood samples were collected from 40 women diagnosed with endometriosis and 40 age-matched healthy controls. Quantitative real-time PCR was conducted to assess the expression levels of the genes, and <i>GAPDH</i> and <i>U6</i> genes were used as internal control genes.</p> Results <p>We observed a notable increase in the expression levels of both <i>hsa-miR-199a-5p</i> and <i>MAP3K11</i> genes, 6.6-times and 9.2-times, respectively, in the patient group compared with healthy controls. Despite increased expression levels of the studied genes, a weak, non-significant negative correlation was observed between them (<i>r</i> = − 0.15).</p> Conclusions <p>These results indicate co-upregulation of both genes in PBMCs, suggesting that their interaction may involve an indirect or post-transcriptional relationship between hsa-miR-199a-5p and <i>MAP3K11</i>. Overall, the findings support that dysregulated circulating miRNAs and their target genes may be associated with endometriosis.</p>

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Dysregulation of hsa-miR-199a-5p and MAP3K11 in Endometriosis: An Association Study

  • 期刊论文

  • 发表日期：

    2026年7月13日

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  • Mahdis Saffarhamidi,
  • Soheila Talesh Sasani,
  • Fereshteh Fakor,
  • Zivar Salehi

摘要

Background

Endometriosis is a gynecological disorder that affects a large number of women worldwide, with several complications including chronic pelvic pain, infertility, and reduced health. Considering the MAPK signaling pathway is involved in endometriosis, we examined the hsa-miR-199a-5p and its predicted target gene, MAP3K11, expression levels to investigate an association between their concurrent dysregulation and endometriosis and to assess their expression changes in peripheral blood mononuclear cells (PBMCs).

Methods

The sequences of hsa-miR-199a-5p and its target gene, MAP3K11, were obtained by using TargetScan and miRDB databases. Blood samples were collected from 40 women diagnosed with endometriosis and 40 age-matched healthy controls. Quantitative real-time PCR was conducted to assess the expression levels of the genes, and GAPDH and U6 genes were used as internal control genes.

Results

We observed a notable increase in the expression levels of both hsa-miR-199a-5p and MAP3K11 genes, 6.6-times and 9.2-times, respectively, in the patient group compared with healthy controls. Despite increased expression levels of the studied genes, a weak, non-significant negative correlation was observed between them (r = − 0.15).

Conclusions

These results indicate co-upregulation of both genes in PBMCs, suggesting that their interaction may involve an indirect or post-transcriptional relationship between hsa-miR-199a-5p and MAP3K11. Overall, the findings support that dysregulated circulating miRNAs and their target genes may be associated with endometriosis.