<p>Reactive dyes are most significant for cotton for their diverse vivid hues, superior colour-fastness, and easy applications. Considering exhaust reactive dyeing, wet-on-wet or wet-on-dry options are available which may differ in results due to pre-swelling of cotton for wet-on-wet dyeing. However, no information is available regarding this in the literature. So, purpose of this study is to address this gap, especially the influence on kinetics of cotton dyeing. To do so, a replicated experimental run was developed utilizing design expert. The data analysis with design expert and excel, using the ANOVA test, revealed that influence of wet-on-wet dyeing on kinetics is not statistically significant. However, it was clearly and consistently observed that dye absorption for wet-on-wet dyeing was lower indicated by dye concentration in fibre and K/S curves.</p>