<p><i>Euphorbia hirta</i> is an important ethnomedicinal herb traditionally used to manage respiratory, gastrointestinal, and infectious diseases. This study evaluates its elemental profile, phytochemical composition, antimicrobial efficacy, and the influence of soil characteristics on its bioactive potential. Elemental concentrations were determined using Atomic Absorption Spectroscopy (AAS), phytochemicals were identified using standard qualitative protocols, and antimicrobial activity was assessed using the agar well-diffusion method against selected bacterial and fungal pathogens. Soil samples were analysed for physicochemical and nutrient parameters. Results showed that essential metals such as zinc, manganese, and iron were within safe limits, while nickel and chromium levels were moderately elevated. Phytochemical screening revealed the presence of tannins, flavonoids, saponins, glycosides, terpenoids, alkaloids, phenols, steroids, and phlobatannins. Strong antibacterial activity was exhibited against Bacillus subtilis, E. coli, and Enterococcus faecalis, while antifungal activity was observed against Penicillium chrysogenum and Aspergillus fumigatus. Soil characteristics were favourable for medicinal plant growth and nutrient uptake. Overall, <i>Euphorbia hirta</i> demonstrates significant therapeutic potential with safe metal content and rich bioactive composition, supporting its traditional and modern medicinal use.</p>