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<p>Phylogenetic analysis serves as a powerful approach to elucidate the evolutionary relationships among diverse organisms. Although ribosomal RNA (rRNA) genes are widely used as phylogenetic markers, their greater similarity among closely related taxa necessitate the exploration of alternative molecular markers. The sequences of heat-shock proteins (HSPs) have shown to be highly conserved across organisms and thus may serve as markers for phylogenetic studies. Multiple studies proved HSPs as better marker when compared to 16S rRNA. Therefore, to better understand the evolutionary aspects using chaperones as markers among species of different kingdoms, we have performed phylogenetic analysis and analyzed multiple sequence alignment of HSP70 and HSP90 sequences. The current study utilized a total of 715 HSP70 and 1261 HSP90 protein sequences to deduce the phylogenetic relationship of eubacteria and eukaryotes. Our analysis displayed separate prokaryotic and eukaryotic branches in both HSP70 and HSP90 trees. Notably, the phyla Streptophyta and Chlorophyta of plantae kingdom were found to cluster together in both the phylogenetic trees reflecting their monophyly. Further, several species showed close clustering consistent with previous reports, while those exhibiting divergence also conformed with earlier studies, indicating the potential of HSP70 and HSP90 as phylogenetic markers. In addition, multiple sequence alignment of HSP70 and HSP90 proteins revealed signature consensus sequences that may serve as target regions for barcoding analysis of closely related species. Taken together, our study indicates HSP70 and HSP90 as additional pan-kingdom phylogenetic markers that can be used along with traditional kingdom-specific markers.</p>

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Analysis of HSP70 and HSP90 as Potential Phylogenetic Markers

  • 期刊论文

  • 发表日期：

    2025年12月6日

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  • Shravanthi Ravula,
  • Qudrathulla Khan Quadri Mohammed,
  • Nidhi Singh Ranawat,
  • Suresh Reddy Mittapalli,
  • Shravan Kumar Gunda,
  • Rama Krishna Kancha

摘要

Phylogenetic analysis serves as a powerful approach to elucidate the evolutionary relationships among diverse organisms. Although ribosomal RNA (rRNA) genes are widely used as phylogenetic markers, their greater similarity among closely related taxa necessitate the exploration of alternative molecular markers. The sequences of heat-shock proteins (HSPs) have shown to be highly conserved across organisms and thus may serve as markers for phylogenetic studies. Multiple studies proved HSPs as better marker when compared to 16S rRNA. Therefore, to better understand the evolutionary aspects using chaperones as markers among species of different kingdoms, we have performed phylogenetic analysis and analyzed multiple sequence alignment of HSP70 and HSP90 sequences. The current study utilized a total of 715 HSP70 and 1261 HSP90 protein sequences to deduce the phylogenetic relationship of eubacteria and eukaryotes. Our analysis displayed separate prokaryotic and eukaryotic branches in both HSP70 and HSP90 trees. Notably, the phyla Streptophyta and Chlorophyta of plantae kingdom were found to cluster together in both the phylogenetic trees reflecting their monophyly. Further, several species showed close clustering consistent with previous reports, while those exhibiting divergence also conformed with earlier studies, indicating the potential of HSP70 and HSP90 as phylogenetic markers. In addition, multiple sequence alignment of HSP70 and HSP90 proteins revealed signature consensus sequences that may serve as target regions for barcoding analysis of closely related species. Taken together, our study indicates HSP70 and HSP90 as additional pan-kingdom phylogenetic markers that can be used along with traditional kingdom-specific markers.