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<p>Brinjal (Solanum melongena L.)&#xa0;and tomato (Solanum lycopersicum L.)&#xa0;cultivation experiences heavy losses due to attack of a number of pests. Among the various pathogens including <i>Ralstonia solanacearum,</i> an important soil-borne pathogens causing diseases with severe yield loss up to 10–90%. The destructiveness of the pathogen is attributed because of its broad host range and capability to survive in soil for a long duration, existence of different strains, etc. During this study, a typical bacterial wilt symptom was observed in brinjals and tomato in four villages of East Sikkim, namely Assam Linzay, Phadamchen, Sajong and Singtam—a major vegetable growing region of the state. In this study, morpho-molecular characterization and identification of <i>R. solanacearum</i> isolated from diseased brinjal and tomato cultivated in Sikkim have been successfully demonstrated. At first, diseased plants were detected based on wilting symptoms and ooze test. Pathogen isolation was carried out following the culture of smear of stem segments of the diseased plants on the Casmino acid, Peptone, Glucose Agar (CPGA) medium. Morphological, biochemical, microscopic and molecular characterization confirmed the isolates as <i>R. solanacearum</i>, race 1, biovar -3, and phylotypes-I (Asiaticum). BLASTn in the NCBI database of the sequence data showed 98–100% similarity to <i>R. solanacearum</i> with accession number OM049425.1, and OM108213.1 for brinjal and tomato, respectively, which is alarming for vegetable cultivation in organic Sikkim.</p>

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Ralstonia solanacearum Isolated from Diseased Brinjal and Tomato: A Potential Threat to Vegetable Cultivation in Sikkim, India

  • 期刊论文

  • 发表日期：

    2025年2月27日

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  • Bahadur Singh Bamaniya,
  • Tshering Lhamu Sherpa,
  • Mushineni Ashajyothi,
  • Laki Dem Bhutia,
  • Rajesh Kumar,
  • Manju Rana,
  • Niladri Bag

摘要

Brinjal (Solanum melongena L.) and tomato (Solanum lycopersicum L.) cultivation experiences heavy losses due to attack of a number of pests. Among the various pathogens including Ralstonia solanacearum, an important soil-borne pathogens causing diseases with severe yield loss up to 10–90%. The destructiveness of the pathogen is attributed because of its broad host range and capability to survive in soil for a long duration, existence of different strains, etc. During this study, a typical bacterial wilt symptom was observed in brinjals and tomato in four villages of East Sikkim, namely Assam Linzay, Phadamchen, Sajong and Singtam—a major vegetable growing region of the state. In this study, morpho-molecular characterization and identification of R. solanacearum isolated from diseased brinjal and tomato cultivated in Sikkim have been successfully demonstrated. At first, diseased plants were detected based on wilting symptoms and ooze test. Pathogen isolation was carried out following the culture of smear of stem segments of the diseased plants on the Casmino acid, Peptone, Glucose Agar (CPGA) medium. Morphological, biochemical, microscopic and molecular characterization confirmed the isolates as R. solanacearum, race 1, biovar -3, and phylotypes-I (Asiaticum). BLASTn in the NCBI database of the sequence data showed 98–100% similarity to R. solanacearum with accession number OM049425.1, and OM108213.1 for brinjal and tomato, respectively, which is alarming for vegetable cultivation in organic Sikkim.