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<p>Several diseases have been linked to oxidative stress including cancer, cardiovascular, neurodegenerative, diabetes, and process of aging. This study aimed at evaluating the effect of ascorbic acid on survival rate and oxidative stress markers in <i>Drosophila melanogaster</i>. A total of 360 flies were divided into four groups with each group having 90 flies (30 flies in triplicate): Group 1 (control), group 2 (H<sub>2</sub>O<sub>2</sub> induced), group 3 (treatment group), and group 4 (ascorbic acid only). Stress was induced in the flies, and 20&#xa0;mM ascorbic acid was used to enrich the flies’ diet, while the mortality rate was recorded at the onset of the induced stress. The induction of stress to the flies significantly (<i>p</i> ≤ 0.05) decreases the survival rate, GSH, total protein, and total thiol levels, while the SOD, CAT, and MDA were significantly increased (<i>p</i> ≤ 0.05). However, treatment with ascorbic acid was found to increase the survival of the flies and modulate the oxidative stress biomarkers in <i>D. melanogaster</i>. Molecular docking studies indicate that ascorbic acid bind to the pocket of Keap1, which may release the Nrf2, thus, facilitating its binding to the antioxidant response element and subsequent activation of antioxidant enzymes. Ascorbic acid is effective in increasing the survival rate and modulating the oxidative stress biomarkers of <i>D. melanogaster</i> exposed to H<sub>2</sub>O<sub>2.</sub></p>

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Ascorbic Acid Increases Survival Rate and Decreases Oxidative Stress via Possible Interaction with Nrf2-Keap1 in Drosophila melanogaster

  • 期刊论文

  • 发表日期：

    2025年6月20日

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  • Idris Zubairu Sadiq,
  • Babangida Sanusi Katsayal,
  • Rashidatu Abdulazeez,
  • Yakubu Fatimat Omengwu,
  • Odunola Safiyyah Adedoyin,
  • Abdullahi Garba Usman

摘要

Several diseases have been linked to oxidative stress including cancer, cardiovascular, neurodegenerative, diabetes, and process of aging. This study aimed at evaluating the effect of ascorbic acid on survival rate and oxidative stress markers in Drosophila melanogaster. A total of 360 flies were divided into four groups with each group having 90 flies (30 flies in triplicate): Group 1 (control), group 2 (H2O2 induced), group 3 (treatment group), and group 4 (ascorbic acid only). Stress was induced in the flies, and 20 mM ascorbic acid was used to enrich the flies’ diet, while the mortality rate was recorded at the onset of the induced stress. The induction of stress to the flies significantly (p ≤ 0.05) decreases the survival rate, GSH, total protein, and total thiol levels, while the SOD, CAT, and MDA were significantly increased (p ≤ 0.05). However, treatment with ascorbic acid was found to increase the survival of the flies and modulate the oxidative stress biomarkers in D. melanogaster. Molecular docking studies indicate that ascorbic acid bind to the pocket of Keap1, which may release the Nrf2, thus, facilitating its binding to the antioxidant response element and subsequent activation of antioxidant enzymes. Ascorbic acid is effective in increasing the survival rate and modulating the oxidative stress biomarkers of D. melanogaster exposed to H2O2.